PE Anti-CD30 antibody [Ber-H8] (ab234273)
![Flow Cytometry - PE Anti-CD30 antibody [Ber-H8] (ab234273)](https://www.abcam.com/ps/products/234/ab234273/Images/ab234273-308355-ab234273.png "Flow Cytometry - PE Anti-CD30 antibody [Ber-H8] (ab234273)")
Key features and details
- PE Mouse monoclonal [Ber-H8] to CD30
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
PE Anti-CD30 antibody [Ber-H8]
See all CD30 primary antibodies -
Description
PE Mouse monoclonal [Ber-H8] to CD30 -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
The details of the immunogen for this antibody are not available.
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Epitope
The mouse monoclonal antibody Ber-H8 recognizes the extracellular part of CD30. -
Positive control
- Flow Cytometry: K562 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
ab234273 is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. -
Clonality
Monoclonal -
Clone number
Ber-H8 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 10µl for 106 cells.
(or 100 μl of whole blood). |
| Notes |
|---|
|
Flow Cyt
Use 10µl for 106 cells. (or 100 μl of whole blood). |
Target
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Function
Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B. -
Sequence similarities
Contains 6 TNFR-Cys repeats. -
Post-translational
modificationsPhosphorylated on serine and tyrosine residues. -
Cellular localization
Cytoplasm and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 943 Human
- Omim: 153243 Human
- SwissProt: P28908 Human
- Unigene: 1314 Human
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Alternative names
- CD 30 antibody
- CD30 antibody
- CD30 antigen antibody
see all
Images
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Flow Cytometry - PE Anti-CD30 antibody [Ber-H8] (ab234273)
Flow cytometric analysis of K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells labeling CD30 with ab234273 (red) compared to a negative control (black). Surface staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab234273 has not yet been referenced specifically in any publications.
