Key features and details
- PE Mouse monoclonal [WM53] to CD33
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Product namePE Anti-CD33 antibody [WM53]
See all CD33 primary antibodies
DescriptionPE Mouse monoclonal [WM53] to CD33
ConjugationPE. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Tissue, cells or virus corresponding to CD33. Human AML cells.
- Flow cyt: Human peripheral blood.
Storage instructionsShipped at 4°C. Store at +4°C. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.0975% Sodium azide
Concentration information loading...
Purification notesThe purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
- APC Anti-CD33 antibody [WM53] (ab118967)
- PerCP Anti-CD33 antibody [WM53] (ab124531)
- APC/Cy7® Anti-CD33 antibody [WM53] (ab206634)
- Anti-CD33 antibody [WM53] - BSA and Azide free (ab213050)
- PE/Cy5® Anti-CD33 antibody [WM53] (ab233448)
- FITC Anti-CD33 antibody [WM53] (ab234216)
- PE/Cy7® Anti-CD33 antibody [WM53] (ab239310)
- Anti-CD33 antibody [WM53] - BSA and Azide free (ab252263)
- Anti-CD33 antibody [WM53] (ab30371)
Our Abpromise guarantee covers the use of ab233577 in the following tested applications.
|Flow Cyt||Use 10µl for 106 cells.
Or 10 µl reagent / 100 µl of whole blood.
FunctionPutative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
Tissue specificityMonocytic/myeloid lineage cells.
Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
DomainContains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
modificationsPhosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6.
Cellular localizationCell membrane.
- Information by UniProt
- CD 33 antibody
- CD33 antibody
- CD33 antigen (gp67) antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233577 has not yet been referenced specifically in any publications.