Key features and details
- PE Mouse monoclonal [4H11(APG)] to CD34, prediluted
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Product namePE Anti-CD34 antibody [4H11(APG)], prediluted
See all CD34 primary antibodies
DescriptionPE Mouse monoclonal [4H11(APG)] to CD34, prediluted
ConjugationPE. Ex: 488nm, Em: 575nm
SpecificityThe antibody reacts with Class III epitope on CD34 antibody. It completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Tissue/ cell preparation (permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia).
- CML cell line MOLM-7.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituents: PBS, BSA
Concentration information loading...
PurityProtein G purified
Purification notesConjugate purified by size-exclusion chromatography.
Our Abpromise guarantee covers the use of ab18228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 20µl for 106 cells.
A cell suspension volume of 100 µl is recommended.
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionPossible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Tissue specificitySelectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
Sequence similaritiesBelongs to the CD34 family.
Developmental stageOn early hematopoietic progenitor cells.
Phosphorylated on serine residues by PKC.
- Information by UniProt
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
ab18228 has been referenced in 2 publications.
- Sun KT et al. Reciprocal regulation of ?-globin expression by exo-miRNAs: Relevance to ?-globin silencing in ß-thalassemia major. Sci Rep 7:202 (2017). PubMed: 28303002
- Castro FV et al. 5T4 oncofetal antigen is expressed in high risk of relapse childhood pre-B acute lymphoblastic leukemia and is associated with a more invasive and chemotactic phenotype. Leukemia 26:1487-98 (2012). Flow Cyt ; Human . PubMed: 22266911