PE Anti-CD34 antibody [ICO-115] (ab187284)
![Flow Cytometry - PE Anti-CD34 antibody [ICO-115] (ab187284)](https://www.abcam.com/ps/products/187/ab187284/Images/ab187284-219613-anti-cd34-antibody-ico-115-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-CD34 antibody [ICO-115] (ab187284)")
Key features and details
- PE Mouse monoclonal [ICO-115] to CD34
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
PE Anti-CD34 antibody [ICO-115]
See all CD34 primary antibodies -
Description
PE Mouse monoclonal [ICO-115] to CD34 -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD34. Blast cells of a chronic myeloid leukemia patient.
Database link: P28906 -
Positive control
- KG-1 cells, Tonsil, or Angiosarcoma
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C or -20°C. Store In the Dark. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
ICO-115 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 5µl for 106 cells.
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt
Use 5µl for 106 cells. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. -
Tissue specificity
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. -
Sequence similarities
Belongs to the CD34 family. -
Developmental stage
On early hematopoietic progenitor cells. -
Post-translational
modificationsHighly glycosylated.
Phosphorylated on serine residues by PKC. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 947 Human
- Omim: 142230 Human
- SwissProt: P28906 Human
- Unigene: 374990 Human
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Alternative names
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
see all
Images
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Flow Cytometry - PE Anti-CD34 antibody [ICO-115] (ab187284)
Surface flow cytometric analysis of CD34 on KG-1 cells using 5 μl ab187284 per one million cells (red) and isotype control antibody (green).
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab187284 has been referenced in 17 publications.
- Babazadeh S et al. Macrophage polarization by MSC-derived CXCL12 determines tumor growth. Cell Mol Biol Lett 26:30 (2021). PubMed: 34174813
- Miyaji T et al. Bone marrow-derived humoral factors suppress oxidative phosphorylation, upregulate TSG-6, and improve therapeutic effects on liver injury of mesenchymal stem cells. J Clin Biochem Nutr 66:213-223 (2020). PubMed: 32523248
- Yue Y et al. Excessive activation of NMDA receptor inhibits the protective effect of endogenous bone marrow mesenchymal stem cells on promoting alveolarization in bronchopulmonary dysplasia. Am J Physiol Cell Physiol 316:C815-C827 (2019). PubMed: 30917030
- Wei ZJ et al. MicroRNA changes of bone marrow-derived mesenchymal stem cells differentiated into neuronal-like cells by Schwann cell-conditioned medium. Neural Regen Res 14:1462-1469 (2019). PubMed: 30964074
- Rao F et al. Exosomes from Human Gingiva-Derived Mesenchymal Stem Cells Combined with Biodegradable Chitin Conduits Promote Rat Sciatic Nerve Regeneration. Stem Cells Int 2019:2546367 (2019). PubMed: 31191669