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  1. Link

    pe-cd34-antibody-qbend--10-ab30377.pdf

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PE Anti-CD34 antibody [QBEND / 10] (ab30377)

  • Datasheet
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Abpromise

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Find out more.

Key features and details

  • PE Mouse monoclonal [QBEND / 10] to CD34
  • Reacts with: Human, Cynomolgus monkey, Rhesus monkey
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Isotype: IgG1

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Recombinant Human CD34 protein (ab126924)
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Anti-CD34 antibody [HPCA1/1171] - BSA and Azide free (ab213058)

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Overview

  • Product name

    PE Anti-CD34 antibody [QBEND / 10]
    See all CD34 primary antibodies
  • Description

    PE Mouse monoclonal [QBEND / 10] to CD34
  • Host species

    Mouse
  • Conjugation

    PE. Ex: 488nm, Em: 575nm
  • Specificity

    QBEND/10 specifically recognises a class II epitope
  • Species reactivity

    Reacts with: Human, Cynomolgus monkey, Rhesus monkey
    Does not react with: Rat, Sheep, Cow, Dog
  • Immunogen

    Human endothelial cell membrane vesicles.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Prepared from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    QBEND / 10
  • Myeloma

    NS0
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Immunology
    • Cell Type Markers
    • CD
    • Myeloid Cells
    • Stem Cells
    • Lineage Markers
    • Mesoderm
    • Stem Cells
    • Mesenchymal Stem Cells
    • Negative Markers
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Cancer
    • Tumor biomarkers
    • Other
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Stem cells
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • HSC markers
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Developmental Biology
    • Lineage specification
    • Mesoderm
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Isotype control

    • PE Mouse IgG1 [B11/6] - Isotype Control (ab91357)
  • Recombinant Protein

    • Recombinant Human CD34 protein (ab126924)

Target

  • Function

    Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
  • Tissue specificity

    Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
  • Sequence similarities

    Belongs to the CD34 family.
  • Developmental stage

    On early hematopoietic progenitor cells.
  • Post-translational
    modifications

    Highly glycosylated.
    Phosphorylated on serine residues by PKC.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P28906 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 101866926 Cynomolgus monkey
    • Entrez Gene: 947 Human
    • Entrez Gene: 713858 Rhesus monkey
    • Omim: 142230 Human
    • SwissProt: P28906 Human
    • Unigene: 374990 Human
    • Alternative names

      • CD34 antibody
      • CD34 antigen antibody
      • CD34 molecule antibody
      • CD34_HUMAN antibody
      • Cluster designation 34 antibody
      • cluster of differentiation 34 antibody
      • Hematopoietic progenitor cell antigen CD34 antibody
      • HPCA1 antibody
      • Mucosialin antibody
      • OTTHUMP00000034733 antibody
      • OTTHUMP00000034734 antibody
      see all

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab30377? Please let us know so that we can cite the reference in this datasheet.

    ab30377 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    Thanks for your email. Further to our conversation on the phone, my supervisor has agreed to choosing 5 antibodies: 4 positive markers and 1 negative marker. The names of the antigens, as well as the clones that may suit the assay best, are tabulated below. It would be best to have them all conjugated with FITC and from a similar IgG subclass; so that I can use only one isotype control. The dye preferred will be FITC, as it works everywhere.
    I would be dearly grateful if you can provide me with the discount codes, for the following
    CD marker
    Abcam cat #
    CD90
    Ab20147
    CD105
    Ab11414
    CD73
    Ab54217
    Many thanks

    Read More

    Abcam community

    Verified customer

    Asked on Apr 19 2012

    Answer

    We unfortunately cannot provide ab20147 and ab54217 as FITC conjugated antibodies because we do not custom conjugate the antibodies.

    We however have the following anti CD90 FITC conjugated antibodies that may be of your interest

    ab226, ab11155, ab25672, ab62009, ab112463, ab112468 and ab124527

    We do not have anti CD73 FITC conjugated antibodies in catalogue.

    ab11415 is the same FITC conjugated version of ab11414.

    Could you choose the antibodies and contact me again.

    You will be happy to know that we do have FITC conjugation kits available in catalogue that can be used to conjugate any antibody customer requires. Please check the datasheet of

    ab10228
    ab102884
    ab102883

    Please let me know which product you are interested in. I will then send you the discount code.

    Read More

    Abcam Scientific Support

    Answered on Apr 19 2012

    Question

    Many thanks for your help. I guess I will try to sort through a multi-species cross-reactive antibody for the time being. I will await your email for tomorrow, and will decide the next steps with my supervisors.
    Again, many thanks for your help
    Regards

    Read More

    Abcam community

    Verified customer

    Asked on Apr 17 2012

    Answer

    Thank you for your email.

    CD271: We have ab8877 which is fully tested with Sheep samples and is guaranteed to work.

    CD34: ab963, ab8536, ab30375, ab30377 are the sheep specific antibodies.

    CD11b: It seems ab75476, ab62817 can cross react due to immunogenic sequence homology.

    CD14: No antibodies

    CD45: No antibodies

    MHC class I: No antibodies

    MHC class II: ab34007

    Please also find attached the Excel list of target specific antibodies we have. The list may help you in selecting the right antibody.

    I hope this info will be helpful. Should you have any other question please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Apr 17 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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