PE Anti-CD34 antibody [QBEND / 10] (ab30377)

Submit a review Q&A (2)

Key features and details

  • PE Mouse monoclonal [QBEND / 10] to CD34
  • Suitable for: Flow Cyt
  • Reacts with: Human, Cynomolgus monkey, Rhesus monkey
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Isotype: IgG1

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
PE Anti-CD34 antibody [EP373Y] (ab223930)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    PE Anti-CD34 antibody [QBEND / 10]
    See all CD34 primary antibodies

  • Description

    PE Mouse monoclonal [QBEND / 10] to CD34

  • Host species

    Mouse

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Specificity

    QBEND/10 specifically recognises a class II epitope

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Human, Cynomolgus monkey, Rhesus monkey
    Does not react with: Rat, Sheep, Cow, Dog

  • Immunogen

    Tissue, cells or virus corresponding to Human CD34. Human endothelial cell membrane vesicles.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab30377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.

Use 10ul of neat preparation to label 10^6 cells in 100 ul. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Notes
Flow Cyt
Use at an assay dependent concentration.

Use 10ul of neat preparation to label 10^6 cells in 100 ul. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

  • Tissue specificity

    Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

  • Sequence similarities

    Belongs to the CD34 family.

  • Developmental stage

    On early hematopoietic progenitor cells.

  • Post-translational
    modifications

    Highly glycosylated.
    Phosphorylated on serine residues by PKC.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD34 antibody
      • CD34 antigen antibody
      • CD34 molecule antibody
      • CD34_HUMAN antibody
      • Cluster designation 34 antibody
      • cluster of differentiation 34 antibody
      • Hematopoietic progenitor cell antigen CD34 antibody
      • HPCA1 antibody
      • Mucosialin antibody
      • OTTHUMP00000034733 antibody
      • OTTHUMP00000034734 antibody
      see all

    References (0)

    Publishing research using ab30377? Please let us know so that we can cite the reference in this datasheet.

    ab30377 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    Thanks for your email. Further to our conversation on the phone, my supervisor has agreed to choosing 5 antibodies: 4 positive markers and 1 negative marker. The names of the antigens, as well as the clones that may suit the assay best, are tabulated below. It would be best to have them all conjugated with FITC and from a similar IgG subclass; so that I can use only one isotype control. The dye preferred will be FITC, as it works everywhere.
    I would be dearly grateful if you can provide me with the discount codes, for the following
    CD marker
    Abcam cat #
    CD90
    Ab20147
    CD105
    Ab11414
    CD73
    Ab54217
    Many thanks

    Read More
    Answer

    We unfortunately cannot provide ab20147 and ab54217 as FITC conjugated antibodies because we do not custom conjugate the antibodies.

    We however have the following anti CD90 FITC conjugated antibodies that may be of your interest

    ab226, ab11155, ab25672, ab62009, ab112463, ab112468 and ab124527

    We do not have anti CD73 FITC conjugated antibodies in catalogue.

    ab11415 is the same FITC conjugated version of ab11414.

    Could you choose the antibodies and contact me again.

    You will be happy to know that we do have FITC conjugation kits available in catalogue that can be used to conjugate any antibody customer requires. Please check the datasheet of

    ab10228
    ab102884
    ab102883

    Please let me know which product you are interested in. I will then send you the discount code.

    Read More

    Question

    Many thanks for your help. I guess I will try to sort through a multi-species cross-reactive antibody for the time being. I will await your email for tomorrow, and will decide the next steps with my supervisors.
    Again, many thanks for your help
    Regards

    Read More
    Answer

    Thank you for your email.

    CD271: We have ab8877 which is fully tested with Sheep samples and is guaranteed to work.

    CD34: ab963, ab8536, ab30375, ab30377 are the sheep specific antibodies.

    CD11b: It seems ab75476, ab62817 can cross react due to immunogenic sequence homology.

    CD14: No antibodies

    CD45: No antibodies

    MHC class I: No antibodies

    MHC class II: ab34007

    Please also find attached the Excel list of target specific antibodies we have. The list may help you in selecting the right antibody.

    I hope this info will be helpful. Should you have any other question please do not hesitate to contact me.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com