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  1. Link

    pe-cd4-antibody-mem-241-ab18282.pdf

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Immunology Adaptive Immunity T Cells CD
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PE Anti-CD4 antibody [MEM-241] (ab18282)

  • Datasheet
  • SDS
Reviews (6)Q&A (6)References (4)

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Flow Cytometry - PE Anti-CD4 antibody [MEM-241] (ab18282)

    Key features and details

    • PE Mouse monoclonal [MEM-241] to CD4
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG1

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    Overview

    • Product name

      PE Anti-CD4 antibody [MEM-241]
      See all CD4 primary antibodies
    • Description

      PE Mouse monoclonal [MEM-241] to CD4
    • Host species

      Mouse
    • Conjugation

      PE. Ex: 488nm, Em: 575nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      CD4 Fusion protein (Human).

    • General notes

      The purified antibody is conjugated with R-Phycoerythrin (R-PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. Do Not Freeze.
    • Storage buffer

      pH: 7.40
      Preservative: 0.097% Sodium azide
      Constituents: PBS, BSA
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      MEM-241
    • Isotype

      IgG1
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Dendritic Cell Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Monocytic Lineage
      • Immunology
      • Adaptive Immunity
      • Regulatory T Cells
      • Immunology
      • Immune System Diseases
      • Antiviral Signaling
      • HIV-related

    Associated products

    • Alternative Versions

      • APC Anti-CD4 antibody [MEM-241] (ab18280)
      • FITC Anti-CD4 antibody [MEM-241] (ab18281)
      • APC/Cy7® Anti-CD4 antibody [MEM241] (ab233298)
      • PE/Cy7® Anti-CD4 antibody [MEM241] (ab233660)
      • Alexa Fluor® 700 Anti-CD4 antibody [MEM-241] (ab270671)
      • Biotin Anti-CD4 antibody [MEM-241] (ab28069)
      • PerCP Anti-CD4 antibody [MEM-241] (ab65951)
    • Isotype control

      • PE Mouse IgG1 [B11/6] - Isotype Control (ab91357)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab18282 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt (5)
    Use at an assay dependent concentration.

    Use 5-20µl for 100µl whole human blood cells.

     

     

     

    ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Notes
    Flow Cyt
    Use at an assay dependent concentration.

    Use 5-20µl for 100µl whole human blood cells.

     

     

     

    ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
    • Sequence similarities

      Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications

      Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
    • Cellular localization

      Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
    • Target information above from: UniProt accession P01730 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 920 Human
      • Omim: 186940 Human
      • SwissProt: P01730 Human
      • Unigene: 631659 Human
      • Alternative names

        • CD 4 antibody
        • CD4 (L3T4) antibody
        • CD4 antibody
        • CD4 antigen (p55) antibody
        • CD4 antigen antibody
        • CD4 molecule antibody
        • CD4 receptor antibody
        • CD4+ Lymphocyte deficiency, included antibody
        • CD4_HUMAN antibody
        • CD4mut antibody
        • L3T4 antibody
        • Leu3 antibody
        • Ly-4 antibody
        • Lymphocyte antigen CD4 antibody
        • MGC165891 antibody
        • OTTHUMP00000238897 antibody
        • p55 antibody
        • T cell antigen T4 antibody
        • T cell antigen T4/LEU3 antibody
        • T cell differentiation antigen L3T4 antibody
        • T cell OKT4 deficiency, included antibody
        • T cell surface antigen T4/Leu 3 antibody
        • T cell surface antigen T4/Leu3 antibody
        • T cell surface glycoprotein CD4 antibody
        • T-cell surface antigen T4/Leu-3 antibody
        • T-cell surface glycoprotein CD4 antibody
        • W3/25 antibody
        • W3/25 antigen antibody
        see all

      Images

      • Flow Cytometry - PE Anti-CD4 antibody [MEM-241] (ab18282)
        Flow Cytometry - PE Anti-CD4 antibody [MEM-241] (ab18282)

        ab18282 staining CD4 in human T cells by Flow Cytometry . 

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (4)

      Publishing research using ab18282? Please let us know so that we can cite the reference in this datasheet.

      ab18282 has been referenced in 4 publications.

      • Kim S  et al. Improved Enumeration of Weakly Fluorescent CD4+ T-lymphocytes by Confining Cells in a Spinning Sample Cartridge with a Helical Minichannel. Micromachines (Basel) 11:N/A (2020). PubMed: 32630535
      • Hernández-Castro JA  et al. Two-level submicron high porosity membranes (2LHPM) for the capture and release of white blood cells (WBCs). Lab Chip 19:589-597 (2019). PubMed: 30648711
      • Dieckmann NM  et al. Munc18-2 is required for Syntaxin 11 Localization on the Plasma Membrane in Cytotoxic T-Lymphocytes. Traffic 16:1330-41 (2015). PubMed: 26771955
      • Seyedi S  et al. The Effects of Isoproterenol and Propranolol on Cytokine Profile Secretion by Cultured Tumor-infiltrating Lymphocytes Derived from Colorectal Cancer Patients. Cell J 13:281-9 (2012). PubMed: 23507624

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review

      Filter by Application

      Filter by Species

      Filter by Ratings

      1-6 of 6 Abreviews

      Flow Cytometry abreview for Anti-CD4 antibody [MEM-241] (Phycoerythrin)

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Rat Cell (Splenocytes)
      Specification
      Splenocytes
      Preparation
      Cell harvesting/tissue preparation method: harvesting of spleen
      Sample buffer: PBS/BSA 1%
      Permeabilization
      No
      Gating Strategy
      alive lymphocytes
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Nov 13 2012

      Flow Cytometry abreview for Anti-CD4 antibody [MEM-241] (Phycoerythrin)

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Mouse Cell (Splenocytes)
      Specification
      Splenocytes
      Preparation
      Cell harvesting/tissue preparation method: harvesting of spleen
      Sample buffer: PBS/BSA 1%
      Permeabilization
      No
      Gating Strategy
      alive lymphocytes
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Nov 13 2012

      Flow Cytometry abreview for Anti-CD4 antibody [MEM-241] (Phycoerythrin)

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Monkey Cell (leucocytes from whole blood)
      Specification
      leucocytes from whole blood
      Preparation
      Cell harvesting/tissue preparation method: after red blood cell lysis
      Sample buffer: PBS/BSA 1%
      Fixation
      Paraformaldehyde
      Permeabilization
      No
      Gating Strategy
      alive lymphocytes
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Nov 13 2012

      Flow Cytometry abreview for Anti-CD4 antibody [MEM-241] (Phycoerythrin)

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Human Cell (leucocytes from whole blood)
      Specification
      leucocytes from whole blood
      Preparation
      Cell harvesting/tissue preparation method: after red blood cell lysis
      Sample buffer: PBS/BSA 1%
      Fixation
      Paraformaldehyde
      Permeabilization
      No
      Gating Strategy
      alive cells
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Nov 12 2012

      Flow Cytometry abreview for Anti-CD4 antibody [MEM-241] (Phycoerythrin)

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Dog Cell (from whole blood)
      Specification
      from whole blood
      Preparation
      Cell harvesting/tissue preparation method: after red blood cell lysis
      Sample buffer: PBS/BSA 1%
      Fixation
      Paraformaldehyde
      Permeabilization
      No
      Gating Strategy
      alive lymphocytes
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Nov 07 2012

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD4 antibody [MEM-241] (Phycoerythrin)

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Thymus)
      Specification
      Thymus
      Fixative
      Paraformaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate buffer PH6.0
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Ruifeng Teng

      Verified customer

      Submitted Apr 30 2012

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