PE Anti-CD4 antibody [MEM-241] (ab18282)
![Flow Cytometry - PE Anti-CD4 antibody [MEM-241] (ab18282)](https://www.abcam.com/ps/products/18/ab18282/Images/ab18282-27928-anti-cd4-antibody-mem-241-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-CD4 antibody [MEM-241] (ab18282)")
Key features and details
- PE Mouse monoclonal [MEM-241] to CD4
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-CD4 antibody [MEM-241]
See all CD4 primary antibodies -
Description
PE Mouse monoclonal [MEM-241] to CD4 -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
CD4 Fusion protein (Human).
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General notes
The purified antibody is conjugated with R-Phycoerythrin (R-PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: PBS, BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
MEM-241 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- APC Anti-CD4 antibody [MEM-241] (ab18280)
- FITC Anti-CD4 antibody [MEM-241] (ab18281)
- APC/Cy7® Anti-CD4 antibody [MEM241] (ab233298)
- PE/Cy7® Anti-CD4 antibody [MEM241] (ab233660)
- Alexa Fluor® 700 Anti-CD4 antibody [MEM-241] (ab270671)
- Biotin Anti-CD4 antibody [MEM-241] (ab28069)
- PerCP Anti-CD4 antibody [MEM-241] (ab65951)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18282 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | (5) |
Use at an assay dependent concentration.
Use 5-20µl for 100µl whole human blood cells.
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. Use 5-20µl for 100µl whole human blood cells.
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 920 Human
- Omim: 186940 Human
- SwissProt: P01730 Human
- Unigene: 631659 Human
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Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Images
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Flow Cytometry - PE Anti-CD4 antibody [MEM-241] (ab18282)
ab18282 staining CD4 in human T cells by Flow Cytometry .
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab18282 has been referenced in 4 publications.
- Kim S et al. Improved Enumeration of Weakly Fluorescent CD4+ T-lymphocytes by Confining Cells in a Spinning Sample Cartridge with a Helical Minichannel. Micromachines (Basel) 11:N/A (2020). PubMed: 32630535
- Hernández-Castro JA et al. Two-level submicron high porosity membranes (2LHPM) for the capture and release of white blood cells (WBCs). Lab Chip 19:589-597 (2019). PubMed: 30648711
- Dieckmann NM et al. Munc18-2 is required for Syntaxin 11 Localization on the Plasma Membrane in Cytotoxic T-Lymphocytes. Traffic 16:1330-41 (2015). PubMed: 26771955
- Seyedi S et al. The Effects of Isoproterenol and Propranolol on Cytokine Profile Secretion by Cultured Tumor-infiltrating Lymphocytes Derived from Colorectal Cancer Patients. Cell J 13:281-9 (2012). PubMed: 23507624