PE Anti-CD40 antibody [5C3] (ab134381)
![Flow Cytometry - PE Anti-CD40 antibody [5C3] (ab134381)](https://www.abcam.com/ps/products/134/ab134381/Images/ab134381-148713-anti-cd40-antibody-5c3-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-CD40 antibody [5C3] (ab134381)")
Key features and details
- PE Mouse monoclonal [5C3] to CD40
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-CD40 antibody [5C3]
See all CD40 primary antibodies -
Description
PE Mouse monoclonal [5C3] to CD40 -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length Human CD40.
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Positive control
- Human peripheral blood cells
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General notes
The 5C3 clone is reported to be used for activation of B cells in in vitro functional assays.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
The 5C3 clone is reported to be used for activation of B cells in in vitro functional assays. -
Clonality
Monoclonal -
Clone number
5C3 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab134381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 5µl for 106 cells. |
Target
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Function
Receptor for TNFSF5/CD40LG. -
Tissue specificity
B-cells and in primary carcinomas. -
Involvement in disease
Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 958 Human
- Omim: 109535 Human
- SwissProt: P25942 Human
- Unigene: 472860 Human
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Alternative names
- AI326936 antibody
- B cell associated molecule CD40 antibody
- B cell surface antigen CD40 antibody
see all
Images
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Flow Cytometry - PE Anti-CD40 antibody [5C3] (ab134381)Flow cytometry analysis of normal Human peripheral blood cells with Mouse IgG1 K Isotype Control PE (blue histogram) or ab134381 at 5 µl (0.015 µg)/ test (purple histogram). Cells in the lymphocyte gate were used for analysis.
Datasheets and documents
References (0)
ab134381 has not yet been referenced specifically in any publications.
