Key features and details
- PE Mouse monoclonal [MEM-263] to CD44
- Suitable for: WB
- Reacts with: Dog, Human, Pig, African green monkey
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Product namePE Anti-CD44 antibody [MEM-263]
See all CD44 primary antibodies
DescriptionPE Mouse monoclonal [MEM-263] to CD44
ConjugationPE. Ex: 488nm, Em: 575nm
Specificityab58754 reacts with the extracellular (N terminal) domain of standard CD44 present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Dog, Human, Pig, African green monkey
Tissue/ cell preparation: Cos 7 cells (African Green Monkey).
- Peripheral blood lymphocytes from human, dog or pig. Kg-1a human acute leukemia cell lysate. JURKAT human leukemia T cell lysate.
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Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA
Concentration information loading...
Purification notesThe conjugate is purified by size exclusion chromatography
Our Abpromise guarantee covers the use of ab58754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).|
FunctionReceptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
Tissue specificityIsoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
Sequence similaritiesContains 1 Link domain.
DomainThe lectin-like LINK domain is responsible for hyaluronan binding.
modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
- Information by UniProt
- LHR antibody
- BA-1 antibody
- CD 44 antibody
ab58754 has been referenced in 2 publications.
- Karakas N et al. Neurons from human mesenchymal stem cells display both spontaneous and stimuli responsive activity. PLoS One 15:e0228510 (2020). PubMed: 32407317
- Linard C et al. Repeated autologous bone marrow-derived mesenchymal stem cell injections improve radiation-induced proctitis in pigs. Stem Cells Transl Med 2:916-27 (2013). Pig . PubMed: 24068742