PE Anti-CD59 antibody [YTH53.1] (ab33165)
Key features and details
- PE Rat monoclonal [YTH53.1] to CD59
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2b
Overview
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Product name
PE Anti-CD59 antibody [YTH53.1]
See all CD59 primary antibodies -
Description
PE Rat monoclonal [YTH53.1] to CD59 -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
This antibody reacts with CD59 transfectants. -
Species reactivity
Reacts with: Human -
Immunogen
Human Peripheral Blood T cells.
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General notes
This clone enhances antibody mediated lysis by homologous complement and reacts with GPI linked antigen of 18-20kD size which is broadly expressed on human leucocytes and erythrocytes.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purification notes
ab33165 was purified by ion exchange chromatography. -
Primary antibody notes
This clone enhances antibody mediated lysis by homologous complement and reacts with GPI linked antigen of 18-20kD size which is broadly expressed on human leucocytes and erythrocytes. -
Clonality
Monoclonal -
Clone number
YTH53.1 -
Myeloma
Y3/Ag1.2.3 -
Isotype
IgG2b -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Target
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Function
Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. -
Involvement in disease
Defects in CD59 are the cause of CD59 deficiency (CD59D) [MIM:612300]. -
Sequence similarities
Contains 1 UPAR/Ly6 domain. -
Post-translational
modificationsN- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.
Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes. -
Cellular localization
Cell membrane. Secreted. Soluble form found in a number of tissues. - Information by UniProt
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Database links
- Entrez Gene: 966 Human
- Omim: 107271 Human
- SwissProt: P13987 Human
- Unigene: 278573 Human
- Unigene: 709466 Human
- Unigene: 710641 Human
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Alternative names
- 16.3A5 antibody
- 1F5 antibody
- 1F5 antigen antibody
see all
Datasheets and documents
References (0)
ab33165 has not yet been referenced specifically in any publications.
