PE Anti-CD62L antibody [LT-TD180] (ab77117)
![Flow Cytometry - PE Anti-CD62L antibody [LT-TD180] (ab77117)](https://www.abcam.com/ps/products/77/ab77117/Images/ab77117-278858-anti-cd62l-antibody-lt-td180-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-CD62L antibody [LT-TD180] (ab77117)")
Key features and details
- PE Mouse monoclonal [LT-TD180] to CD62L
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-CD62L antibody [LT-TD180]
See all CD62L primary antibodies -
Description
PE Mouse monoclonal [LT-TD180] to CD62L -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
Reacts with CD62L (L selectin), a 74-95 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Peripheral blood leukocytes.
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Positive control
- Human blood cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, 0.2% PBS
0.2% BSA (high-grade protease free) -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
The purified antibody (protein A (G) affinity chromatography before conjugation) is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. -
Clonality
Monoclonal -
Clone number
LT-TD180 -
Isotype
IgG1 -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab77117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. -
Tissue specificity
Expressed in B cell lines and T lymphocytes. -
Sequence similarities
Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6402 Human
- Omim: 153240 Human
- SwissProt: P14151 Human
- Unigene: 728756 Human
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Alternative names
- A.11 antibody
- AI528707 antibody
- CD62 antigen ligand antibody
see all
Images
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Flow Cytometry - PE Anti-CD62L antibody [LT-TD180] (ab77117)Flow Cytometry analysis of Human peripheral blood cells labeling CD62L with Anti-CD62L antibody [LT-TD180] (Phycoerythrin) (ab77117).
References (3)
ab77117 has been referenced in 3 publications.
- Linnebacher M et al. Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260(FTO). J Biomed Biotechnol 2010:841451 (2010). PubMed: 20339516
- Kuttruff S et al. NKp80 defines and stimulates a reactive subset of CD8 T cells. Blood 113:358-69 (2009). PubMed: 18922855
- Simone R et al. The frequency of regulatory CD3+CD8+CD28- CD25+ T lymphocytes in human peripheral blood increases with age. J Leukoc Biol 84:1454-61 (2008). PubMed: 18780874
