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  1. Link

    pe-cd63-antibody-mem-259-prediluted-ab18236.pdf

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PE Anti-CD63 antibody [MEM-259], prediluted (ab18236)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (1)

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Flow Cytometry - PE Anti-CD63 antibody [MEM-259], prediluted (ab18236)

    Key features and details

    • PE Mouse monoclonal [MEM-259] to CD63, prediluted
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG1

    Overview

    • Product name

      PE Anti-CD63 antibody [MEM-259], prediluted
      See all CD63 primary antibodies
    • Description

      PE Mouse monoclonal [MEM-259] to CD63, prediluted
    • Host species

      Mouse
    • Conjugation

      PE. Ex: 488nm, Em: 575nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Tissue/ cell preparation (Human T cell line HPB-ALL).

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.40
      Preservative: 0.097% Sodium azide
      Constituents: PBS, BSA
    • Concentration information loading...
    • Purification notes

      The conjugate is purified by size-exclusion chromatography.
    • Clonality

      Monoclonal
    • Clone number

      MEM-259
    • Isotype

      IgG1
    • Research areas

      • Cardiovascular
      • Blood
      • Platelets
      • Immunology
      • Cell Type Markers
      • CD
      • Platelets
      • Tags & Cell Markers
      • Cell Type Markers
      • Tumor Associated
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Cell adhesion
      • Other
      • Cancer
      • Tumor biomarkers
      • Tumor antigens

    Associated products

    • Alternative Versions

      • Biotin Anti-CD63 antibody [MEM-259] (ab134331)
      • FITC Anti-CD63 antibody [MEM-259] (ab18235)
      • PE/Cy5® Anti-CD63 antibody [MEM-259] (ab234251)
      • PerCP Anti-CD63 antibody [MEM-259] (ab77227)
      • Anti-CD63 antibody [MEM-259] (ab8219)
    • Isotype control

      • PE Mouse IgG1 [B11/6] - Isotype Control (ab91357)

    Applications

    Our Abpromise guarantee covers the use of ab18236 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 20µl for 106 cells.

    20 µl for a 100 µl sample is recommended.

     

     

     

     

    ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
    • Tissue specificity

      Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
    • Sequence similarities

      Belongs to the tetraspanin (TM4SF) family.
    • Cellular localization

      Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
    • Target information above from: UniProt accession P08962 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 967 Human
      • Omim: 155740 Human
      • SwissProt: P08962 Human
      • Unigene: 445570 Human
      • Alternative names

        • Lysosomal associated membrane protein 3 antibody
        • CD 63 antibody
        • CD63 antibody
        • CD63 antigen (melanoma 1 antigen) antibody
        • CD63 antigen antibody
        • CD63 antigen melanoma 1 antigen antibody
        • CD63 molecule antibody
        • CD63_HUMAN antibody
        • gp55 antibody
        • Granulophysin antibody
        • LAMP 3 antibody
        • LAMP-3 antibody
        • LAMP3 antibody
        • LIMP antibody
        • Lysosomal-associated membrane protein 3 antibody
        • Lysosome associated membrane glycoprotein 3 antibody
        • Lysosome-associated membrane glycoprotein 3 antibody
        • Mast cell antigen AD1 antibody
        • ME491 antibody
        • Melanoma 1 antigen antibody
        • Melanoma associated antigen ME491 antibody
        • Melanoma associated antigen MLA1 antibody
        • Melanoma-associated antigen ME491 antibody
        • MGC72893 antibody
        • MLA 1 antibody
        • MLA1 antibody
        • NGA antibody
        • Ocular melanoma associated antigen antibody
        • Ocular melanoma-associated antigen antibody
        • OMA81H antibody
        • PTLGP40 antibody
        • Tetraspanin 30 antibody
        • Tetraspanin-30 antibody
        • Tspan 30 antibody
        • Tspan-30 antibody
        • TSPAN30 antibody
        see all

      Images

      • Flow Cytometry - PE Anti-CD63 antibody [MEM-259], prediluted (ab18236)
        Flow Cytometry - PE Anti-CD63 antibody [MEM-259], prediluted (ab18236)

        Flow cytometry analysis of IgE-activated peripheral blood stained with anti-human CD63 (MEM-259) PE. 

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab18236? Please let us know so that we can cite the reference in this datasheet.

      ab18236 has been referenced in 1 publication.

      • Lettau M  et al. Mechanistic peculiarities of activation-induced mobilization of cytotoxic effector proteins in human T cells. Int Immunol 30:215-228 (2018). PubMed: 29373679

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Can you tell me the concentration?

      Read More

      Abcam community

      Verified customer

      Asked on Apr 10 2006

      Answer

      Thank you for your enquiry. The concentration of product ab18236 is 25 ug/ml. I hope this information helps. Please do not hesitate to contact us if you need anything further.

      Read More

      Abcam Scientific Support

      Answered on Apr 12 2006

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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