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Cardiovascular Blood Platelets
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PE Anti-CD63 antibody [MX-49.129.5] (ab205540)

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Flow Cytometry - PE Anti-CD63 antibody [MX-49.129.5] (ab205540)

    Key features and details

    • PE Mouse monoclonal [MX-49.129.5] to CD63
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG1

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    Overview

    • Product name

      PE Anti-CD63 antibody [MX-49.129.5]
      See all CD63 primary antibodies
    • Description

      PE Mouse monoclonal [MX-49.129.5] to CD63
    • Host species

      Mouse
    • Conjugation

      PE. Ex: 488nm, Em: 575nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Full length protein corresponding to Human CD63 aa 1-238.
      Sequence:

      MAVEGGMKCVKFLLYVLLLAFCACAVGLIAVGVGAQLVLSQTIIQGATPG SLLPVVIIAVGVFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAA AIAGYVFRDKVMSEFNNNFRQQMENYPKNNHTASILDRMQADFKCCGAAN YTDWEKIPSMSKNRVPDSCCINVTVGCGINFNEKAIHKEGCVEKIGGWLR KNVLVVAAAALGIAFVEVLGIVFACCLVKSIRSGYEVM


      Database link: P08962
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Flow Cyt: Human PBMC cells.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. Store In the Dark.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituents: 99% PBS, 0.05% BSA
    • Concentration information loading...
    • Purity

      Protein A/G purified
    • Purification notes

      Purified from bioreactor concentrate.
    • Clonality

      Monoclonal
    • Clone number

      MX-49.129.5
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Cardiovascular
      • Blood
      • Platelets
      • Immunology
      • Cell Type Markers
      • CD
      • Platelets
      • Tags & Cell Markers
      • Cell Type Markers
      • Tumor Associated
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Cell adhesion
      • Other
      • Cancer
      • Tumor biomarkers
      • Tumor antigens

    Associated products

    • Alternative Versions

      • Anti-CD63 antibody [MX-49.129.5] (ab193349)
      • Anti-CD63 antibody [MX-49.129.5] - BSA and Azide free (ab213092)
    • Isotype control

      • PE Mouse IgG1 [B11/6] - Isotype Control (ab91357)

    Applications

    Our Abpromise guarantee covers the use of ab205540 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 5µl for 106 cells.

    or 5 µL per 100 µL of whole blood

    Target

    • Function

      This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
    • Tissue specificity

      Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
    • Sequence similarities

      Belongs to the tetraspanin (TM4SF) family.
    • Cellular localization

      Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
    • Target information above from: UniProt accession P08962 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 967 Human
      • Omim: 155740 Human
      • SwissProt: P08962 Human
      • Unigene: 445570 Human
      • Alternative names

        • Lysosomal associated membrane protein 3 antibody
        • CD 63 antibody
        • CD63 antibody
        • CD63 antigen (melanoma 1 antigen) antibody
        • CD63 antigen antibody
        • CD63 antigen melanoma 1 antigen antibody
        • CD63 molecule antibody
        • CD63_HUMAN antibody
        • gp55 antibody
        • Granulophysin antibody
        • LAMP 3 antibody
        • LAMP-3 antibody
        • LAMP3 antibody
        • LIMP antibody
        • Lysosomal-associated membrane protein 3 antibody
        • Lysosome associated membrane glycoprotein 3 antibody
        • Lysosome-associated membrane glycoprotein 3 antibody
        • Mast cell antigen AD1 antibody
        • ME491 antibody
        • Melanoma 1 antigen antibody
        • Melanoma associated antigen ME491 antibody
        • Melanoma associated antigen MLA1 antibody
        • Melanoma-associated antigen ME491 antibody
        • MGC72893 antibody
        • MLA 1 antibody
        • MLA1 antibody
        • NGA antibody
        • Ocular melanoma associated antigen antibody
        • Ocular melanoma-associated antigen antibody
        • OMA81H antibody
        • PTLGP40 antibody
        • Tetraspanin 30 antibody
        • Tetraspanin-30 antibody
        • Tspan 30 antibody
        • Tspan-30 antibody
        • TSPAN30 antibody
        see all

      Images

      • Flow Cytometry - PE Anti-CD63 antibody [MX-49.129.5] (ab205540)
        Flow Cytometry - PE Anti-CD63 antibody [MX-49.129.5] (ab205540)

        Flow cytometry analysis of human PBMC cells labeling CD63 using ab205540 at 5 µL for 106 cells (Red). Black: Cells alone; Green: Isotype control.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab205540? Please let us know so that we can cite the reference in this datasheet.

      ab205540 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Please use the links above to contact us or submit feedback about this product.

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