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  1. Link

    pe-cd68-antibody-fa-11-ab216701.pdf

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Immunology Cell Type Markers CD Myeloid Cells
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PE Anti-CD68 antibody [FA-11] (ab216701)

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Flow Cytometry - PE Anti-CD68 antibody [FA-11] (ab216701)

    Key features and details

    • PE Rat monoclonal [FA-11] to CD68
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG2a

    You may also be interested in

    Protein
    Recombinant human CD68 protein (Fc Chimera Active) (ab214829)

    View more associated products

    Overview

    • Product name

      PE Anti-CD68 antibody [FA-11]
      See all CD68 primary antibodies
    • Description

      PE Rat monoclonal [FA-11] to CD68
    • Host species

      Rat
    • Conjugation

      PE. Ex: 488nm, Em: 575nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      Tissue, cells or virus corresponding to Mouse CD68.

    • Positive control

      • Flow Cyt: Raw264.7 cells
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 1% BSA, PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
    • Clonality

      Monoclonal
    • Clone number

      FA-11
    • Isotype

      IgG2a
    • Research areas

      • Immunology
      • Cell Type Markers
      • CD
      • Myeloid Cells

    Associated products

    • Alternative Versions

      • Alexa Fluor® 405 Anti-CD68 antibody [FA-11] (ab199571)
      • Alexa Fluor® 488 Anti-CD68 antibody [FA-11] (ab201844)
      • Alexa Fluor® 647 Anti-CD68 antibody [FA-11] (ab201845)
      • HRP Anti-CD68 antibody [FA-11] (ab216526)
      • PerCP Anti-CD68 antibody [FA-11] (ab220509)
      • APC Anti-CD68 antibody [FA-11] (ab221251)
      • Anti-CD68 antibody [FA-11] - BSA and Azide free (ab237968)
      • Anti-CD68 antibody [FA-11] (ab53444)
    • Recombinant Protein

      • Recombinant human CD68 protein (Fc Chimera Active) (ab214829)

    Applications

    Our Abpromise guarantee covers the use of ab216701 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt 1/1000.

    The cellular localisation of this product has been verified in ICC/IF.

    Target

    • Function

      Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
    • Tissue specificity

      Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
    • Sequence similarities

      Belongs to the LAMP family.
    • Post-translational
      modifications

      N- and O-glycosylated.
    • Cellular localization

      Cell membrane and Endosome membrane. Lysosome membrane.
    • Target information above from: UniProt accession P34810 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 12514 Mouse
      • SwissProt: P31996 Mouse
      • Unigene: 15819 Mouse
      • Alternative names

        • CD 68 antibody
        • CD68 antibody
        • CD68 antigen antibody
        • CD68 molecule antibody
        • CD68_HUMAN antibody
        • DKFZp686M18236 antibody
        • gp11 antibody
        • Gp110 antibody
        • LAMP4 antibody
        • Macrophage antigen CD68 (microsialin) antibody
        • MACROPHAGE ANTIGEN CD68 antibody
        • Macrosialin antibody
        • SCARD1 antibody
        • Scavenger receptor class D member 1 antibody
        see all

      Images

      • Flow Cytometry - PE Anti-CD68 antibody [FA-11] (ab216701)
        Flow Cytometry - PE Anti-CD68 antibody [FA-11] (ab216701)

        Overlay histogram showing Raw264.7 cells stained with ab216701 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab216701, 1/1000 dilution) for 30 min at 22°C.

        Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

        Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

        This antibody gave a positive signal in Raw264.7 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab216701? Please let us know so that we can cite the reference in this datasheet.

      ab216701 has not yet been referenced specifically in any publications.

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