PE Anti-CD7 antibody [Huly-m2] (ab1160)
Key features and details
- PE Mouse monoclonal [Huly-m2] to CD7
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Overview
-
Product name
PE Anti-CD7 antibody [Huly-m2]
See all CD7 primary antibodies -
Description
PE Mouse monoclonal [Huly-m2] to CD7 -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
This antibody does not react with granulocytes and B lymphocytes. -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to CD7.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
Huly-m2 -
Myeloma
unknown -
Isotype
IgG2a -
Light chain type
unknown -
Research areas
Associated products
-
Isotype control
-
Recombinant Protein
Applications
Application notes
Diagnosis and follow-up of T cell acute lymphoblastic leukemia (T-ALL) andAcute myeloid leukaemia (CD7+ AML)in human lysed whole peripheral blood or mononuclear cells separated by density gradient.
For FACS:
Add 100ml of whole blood to a staining tube.
To this tube add 10ml of CD7 (PE)reagent.
Mix gently and incubate 20-30 minutes at 2-8°C.
Add 2ml of BIO LISANTE(ab1159) at room temperature.
Vortex tube gently (no more then 2 seconds)then incubate for 10 minutes at room temperature in the dark.
Wash cells two timeswith 3ml of PBS.
Resuspend cells in 0.5-1ml of PBS and analyse.
CD7 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.
10µl ofCD7 (PE) is sufficient for labelling of 1x106 cells.
Molecular F/P ratio: 1.0
For FACS:
Add 100ml of whole blood to a staining tube.
To this tube add 10ml of CD7 (PE)reagent.
Mix gently and incubate 20-30 minutes at 2-8°C.
Add 2ml of BIO LISANTE(ab1159) at room temperature.
Vortex tube gently (no more then 2 seconds)then incubate for 10 minutes at room temperature in the dark.
Wash cells two timeswith 3ml of PBS.
Resuspend cells in 0.5-1ml of PBS and analyse.
CD7 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.
10µl ofCD7 (PE) is sufficient for labelling of 1x106 cells.
Molecular F/P ratio: 1.0
Target
-
Function
Not yet known. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 924 Human
- Omim: 186820 Human
- SwissProt: P09564 Human
- Unigene: 36972 Human
-
Alternative names
- CD7 antibody
- CD7 antigen (p41) antibody
- CD7 antigen antibody
see all
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab1160 has been referenced in 2 publications.
- Porwit-MacDonald A et al. BIOMED-1 concerted action report: flow cytometric characterization of CD7+ cell subsets in normal bone marrow as a basis for the diagnosis and follow-up of T cell acute lymphoblastic leukemia (T-ALL). Leukemia 14:816-25 (2000). PubMed: 10803512
- Thurlow PJ et al. A monoclonal antibody detecting a new human T cell antigen, HuLy-m2. Transplantation 38:143-7 (1984). PubMed: 6205484