PE Anti-CD7 antibody [Huly-m2] (ab1160)
Key features and details
- PE Mouse monoclonal [Huly-m2] to CD7
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Overview
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Product name
PE Anti-CD7 antibody [Huly-m2]
See all CD7 primary antibodies -
Description
PE Mouse monoclonal [Huly-m2] to CD7 -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
This antibody does not react with granulocytes and B lymphocytes. -
Species reactivity
Reacts with: Human -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
Huly-m2 -
Myeloma
unknown -
Isotype
IgG2a -
Light chain type
unknown -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
For FACS:
Add 100ml of whole blood to a staining tube.
To this tube add 10ml of CD7 (PE)reagent.
Mix gently and incubate 20-30 minutes at 2-8°C.
Add 2ml of BIO LISANTE(ab1159) at room temperature.
Vortex tube gently (no more then 2 seconds)then incubate for 10 minutes at room temperature in the dark.
Wash cells two timeswith 3ml of PBS.
Resuspend cells in 0.5-1ml of PBS and analyse.
CD7 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.
10µl ofCD7 (PE) is sufficient for labelling of 1x106 cells.
Molecular F/P ratio: 1.0
Target
-
Function
Not yet known. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 924 Human
- Omim: 186820 Human
- SwissProt: P09564 Human
- Unigene: 36972 Human
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Alternative names
- CD7 antibody
- CD7 antigen (p41) antibody
- CD7 antigen antibody
see all
References (2)
ab1160 has been referenced in 2 publications.
- Porwit-MacDonald A et al. BIOMED-1 concerted action report: flow cytometric characterization of CD7+ cell subsets in normal bone marrow as a basis for the diagnosis and follow-up of T cell acute lymphoblastic leukemia (T-ALL). Leukemia 14:816-25 (2000). PubMed: 10803512
- Thurlow PJ et al. A monoclonal antibody detecting a new human T cell antigen, HuLy-m2. Transplantation 38:143-7 (1984). PubMed: 6205484
