PE Anti-CD79a antibody [HM47] (ab177274)

Submit a review Submit a question References (2)
Flow Cytometry (Intracellular) - PE Anti-CD79a antibody [HM47] (ab177274)
  • Flow Cytometry (Intracellular) - PE Anti-CD79a antibody [HM47] (ab177274)
  • Flow Cytometry (Intracellular) - PE Anti-CD79a antibody [HM47] (ab177274)

Key features and details

  • PE Mouse monoclonal [HM47] to CD79a
  • Suitable for: Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Isotype: IgG1

Overview

  • Product name

    PE Anti-CD79a antibody [HM47]
    See all CD79a primary antibodies

  • Description

    PE Mouse monoclonal [HM47] to CD79a

  • Host species

    Mouse

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested applications

    Suitable for: Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to Human CD79a aa 208-222 (intracellular).

  • Positive control

    • Flow Cyt (Intracellular): Human blood.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab177274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra) Use at an assay dependent concentration.

10 μl reagent / 100 μl of whole blood or 106 cells in a suspension.

Target

  • Function

    Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B cells.

  • Tissue specificity

    B-cells.

  • Involvement in disease

    Defects in CD79A are the cause of agammaglobulinemia type 3 (AGM3) [MIM:613501]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. Note=Two different mutations, one at the splice donor site of intron 2 and the other at the splice acceptor site for exon 3, have been identified. Both mutations give rise to a truncated protein.

  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 ITAM domain.

  • Post-translational
    modifications

    Phosphorylated on tyrosine, serine and threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early and essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylation of serine and threonine residues may prevent subsequent tyrosine phosphorylation.

  • Cellular localization

    Cell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.
  • Information by UniProt

  • Database links

    • Alternative names

      • B lymphocyte-specific MB1 protein antibody
      • B-cell antigen receptor complex-associated protein alpha chain antibody
      • CD 79a antibody
      • CD79a antibody
      • CD79a antigen (immunoglobulin-associated alpha) antibody
      • CD79A antigen antibody
      • CD79a molecule, immunoglobulin-associated alpha antibody
      • CD79A_HUMAN antibody
      • Ig alpha antibody
      • Ig-alpha antibody
      • IGA antibody
      • IgM-alpha antibody
      • Immunoglobulin-associated alpha antibody
      • Ly54 antibody
      • MB-1 membrane glycoprotein antibody
      • MB1 antibody
      • Membrane-bound immunoglobulin-associated protein antibody
      • Surface IgM-associated protein antibody
      see all

    Images

    • Flow Cytometry (Intracellular) - PE Anti-CD79a antibody [HM47] (ab177274)
      Flow Cytometry (Intracellular) - PE Anti-CD79a antibody [HM47] (ab177274)
      Flow cytometry analysis of human peripheral blood labeling CD79a using ab177274 at 10 µL/100ul peripheral whole blood. Intracellular staining.
    • Flow Cytometry (Intracellular) - PE Anti-CD79a antibody [HM47] (ab177274)
      Flow Cytometry (Intracellular) - PE Anti-CD79a antibody [HM47] (ab177274)
      Flow cytometry analysis of human peripheral blood labeling CD79a using ab177274 at 10 µL/100ul peripheral whole blood. CD19 is labeled with an Anti-human CD19-PE conjugate at 10 µL/100ul peripheral whole blood. Intracellular staining.
    • Flow Cytometry (Intracellular) - PE Anti-CD79a antibody [HM47] (ab177274)
      Flow Cytometry (Intracellular) - PE Anti-CD79a antibody [HM47] (ab177274)
      Flow cytometry analysis of human peripheral blood labeling CD79a using ab177274 at 10 µL/100ul peripheral whole blood. CD79a-positive B-cells (Red) are separated from CD79-negative neutrophil granulocytes (Black, dashed line). Intracellular staining.

    References (2)

    Publishing research using ab177274? Please let us know so that we can cite the reference in this datasheet.

    ab177274 has been referenced in 2 publications.

    • Pennings I  et al. Effect of donor variation on osteogenesis and vasculogenesis in hydrogel cocultures. J Tissue Eng Regen Med 13:433-445 (2019). PubMed: 30650247
    • Rowart P  et al. Mesenchymal Stromal Cells Accelerate Epithelial Tight Junction Assembly via the AMP-Activated Protein Kinase Pathway, Independently of Liver Kinase B1. Stem Cells Int 2017:9717353 (2017). Flow Cyt ; Dog . PubMed: 28781597

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab177274.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com