PE Anti-CD8 alpha antibody [EP72] (ab25436)
Key features and details
- PE Mouse monoclonal [EP72] to CD8 alpha
- Suitable for: IP, Flow Cyt, IHC-Fr, Purification
- Reacts with: Chicken
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2b
Overview
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Product name
PE Anti-CD8 alpha antibody [EP72]
See all CD8 alpha primary antibodies -
Description
PE Mouse monoclonal [EP72] to CD8 alpha -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
ab25436 recognises alpha chain of chicken CD8. -
Tested applications
Suitable for: IP, Flow Cyt, IHC-Fr, Purificationmore details -
Species reactivity
Reacts with: Chicken -
Immunogen
Tissue, cells or virus corresponding to Chicken CD8 alpha. Chicken splenocytes
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Stabilising agent. -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP72 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
ab91532 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
| IHC-Fr |
Use at an assay dependent concentration.
Acetone fixed. |
|
| Purification |
Use at an assay dependent concentration.
Purification of CD8+ T cells by positive cell sorting. |
| Notes |
|---|
|
IP
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab91532 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
IHC-Fr
Use at an assay dependent concentration. Acetone fixed. |
|
Purification
Use at an assay dependent concentration. Purification of CD8+ T cells by positive cell sorting. |
Target
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Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab25436 has not yet been referenced specifically in any publications.
