PE Anti-CD8 antibody [LT8] (ab28017)

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Flow Cytometry - PE Anti-CD8 antibody [LT8] (ab28017)

    Key features and details

    • PE Mouse monoclonal [LT8] to CD8
    • Suitable for: Flow Cyt
    • Reacts with: Human, Rhesus monkey
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG1

    Overview

    • Product name

      PE Anti-CD8 antibody [LT8]
      See all CD8 primary antibodies

    • Description

      PE Mouse monoclonal [LT8] to CD8

    • Host species

      Mouse

    • Conjugation

      PE. Ex: 488nm, Em: 575nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human, Rhesus monkey

    • Immunogen

      Tissue, cells or virus corresponding to Human CD8. Normal human peripheral blood lymphocytes.

    • General notes

      Purified IgG conjugated to R. Phycoerythrin (RPE).

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

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    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab28017 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use 10µl for 106 cells.

    Or 100µl human whole blood.
    Notes
    Flow Cyt
    Use 10µl for 106 cells.

    Or 100µl human whole blood.

    Target

    • Function

      Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

    • Involvement in disease

      Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.

    • Sequence similarities

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Post-translational
      modifications

      All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.

    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt

    • Database links

      • Form

        CD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.

      • Alternative names

        • CD8 antibody
        • CD8 antigen, alpha polypeptide (p32) antibody
        • CD8a antibody
        • CD8a antigen antibody
        • CD8a molecule antibody
        • CD8A_HUMAN antibody
        • Leu2 antibody
        • Leu2 T lymphocyte antigen antibody
        • MAL antibody
        • OKT8 T cell antigen antibody
        • p32 antibody
        • T cell antigen Leu2 antibody
        • T cell co receptor antibody
        • T-cell surface glycoprotein CD8 alpha chain antibody
        • T-lymphocyte differentiation antigen T8/Leu-2 antibody
        • T8 T cell antigen antibody
        see all

      Images

      • Flow Cytometry - PE Anti-CD8 antibody [LT8] (ab28017)
        Flow Cytometry - PE Anti-CD8 antibody [LT8] (ab28017)

      References (1)

      Publishing research using ab28017? Please let us know so that we can cite the reference in this datasheet.

      ab28017 has been referenced in 1 publication.

      • Elkord E  et al. Differential CTLs specific for prostate-specific antigen in healthy donors and patients with prostate cancer. Int Immunol 17:1315-25 (2005). PubMed: 16141246

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