PE Anti-CD8 beta antibody [341] (ab95526)
![Flow Cytometry - PE Anti-CD8 beta antibody [341] (ab95526)](https://www.abcam.com/ps/products/95/ab95526/Images/ab95526-109811-anti-cd8-antibody-341-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-CD8 beta antibody [341] (ab95526)")
Key features and details
- PE Mouse monoclonal [341] to CD8 beta
- Suitable for: Flow Cyt
- Reacts with: Rat
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-CD8 beta antibody [341]
See all CD8 beta primary antibodies -
Description
PE Mouse monoclonal [341] to CD8 beta -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Tissue, cells or virus corresponding to Rat CD8 beta. Rat/mouse T-cell hybrids expressing CD8 beta.
Database link: P05541 -
Positive control
- Rat splenocytes
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General notes
Excitation: 488 nm Emission: 575 nm.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
341 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab95526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.25µg for 105-8 cells. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. -
Tissue specificity
Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPhosphorylated as a consequence of T-cell activation. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 24931 Rat
- SwissProt: P05541 Rat
- Unigene: 10330 Rat
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Alternative names
- CD8b antigen antibody
- CD_antigen=CD8b antibody
- CD8 antigen 37 kDa chain antibody
see all
Images
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Flow Cytometry - PE Anti-CD8 beta antibody [341] (ab95526)
Staining of rat splenocytes with 0.125 µg of Mouse IgG1 kappa Isotype Control PE (open histogram) or 0.125 µg of ab95526 (filled histogram). Cells in the lymphocyte gate were used for analysis.
Datasheets and documents
References (0)
ab95526 has not yet been referenced specifically in any publications.
