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Immunology Adaptive Immunity B Cells CD
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PE Anti-CD80 antibody [MEM-233], prediluted (ab69778)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (2)

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Flow Cytometry - PE Anti-CD80 antibody [MEM-233], prediluted (ab69778)

    Key features and details

    • PE Mouse monoclonal [MEM-233] to CD80, prediluted
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG1

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    Overview

    • Product name

      PE Anti-CD80 antibody [MEM-233], prediluted
      See all CD80 primary antibodies
    • Description

      PE Mouse monoclonal [MEM-233] to CD80, prediluted
    • Host species

      Mouse
    • Conjugation

      PE. Ex: 488nm, Em: 575nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      CD80 fusion protein

    • General notes

      The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.4
      Preservative: 0.097% Sodium azide
      Constituents: 0.2% BSA, PBS
    • Concentration information loading...
    • Purity

      Size exclusion
    • Clonality

      Monoclonal
    • Clone number

      MEM-233
    • Isotype

      IgG1
    • Research areas

      • Immunology
      • Adaptive Immunity
      • B Cells
      • CD
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Dendritic Cell Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Monocytic Lineage

    Associated products

    • Alternative Versions

      • FITC Anti-CD80 antibody [MEM-233] (ab18279)
      • PE/Cy5® Anti-CD80 antibody [MEM-233] (ab233572)
      • PE/Cy7® Anti-CD80 antibody [MEM-233] (ab234229)
      • Alexa Fluor® 700 Anti-CD80 antibody [MEM-233] (ab270668)
      • APC Anti-CD80 antibody [MEM-233] (ab27554)
      • Anti-CD80 antibody [MEM-233] (ab8239)
      • PerCP Anti-CD80 antibody [MEM-233] (ab82410)
    • Isotype control

      • PE Mouse IgG1 [B11/6] - Isotype Control (ab91357)
    • Recombinant Protein

      • Recombinant Human CD80 protein (ab131745)

    Applications

    Our Abpromise guarantee covers the use of ab69778 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
  • Application notes
    Flow Cyt: Use 20µl for 106 cells or 100µl whole blood.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
    • Tissue specificity

      Expressed on activated B-cells, macrophages and dendritic cells.
    • Sequence similarities

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P33681 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 941 Human
      • Omim: 112203 Human
      • SwissProt: P33681 Human
      • Unigene: 838 Human
      • Alternative names

        • Activation B7-1 antigen antibody
        • B lymphocyte activation antigen B7 antibody
        • B7 antibody
        • B7-1 antibody
        • B7-1 antigen antibody
        • B7.1 antibody
        • BB1 antibody
        • CD28 antigen ligand 1 antibody
        • CD28LG antibody
        • CD28LG1 antibody
        • CD80 antibody
        • CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) antibody
        • CD80 antigen antibody
        • CD80 molecule antibody
        • CD80_HUMAN antibody
        • Costimulatory factor CD80 antibody
        • costimulatory molecule variant IgV-CD80 antibody
        • CTLA-4 counter-receptor B7.1 antibody
        • LAB7 antibody
        • T-lymphocyte activation antigen CD80 antibody
        see all

      Images

      • Flow Cytometry - PE Anti-CD80 antibody [MEM-233], prediluted (ab69778)
        Flow Cytometry - PE Anti-CD80 antibody [MEM-233], prediluted (ab69778)
        Surface staining of CD80-transfected P815 cells with anti-human CD80 (MEM-233) PE.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (2)

      Publishing research using ab69778? Please let us know so that we can cite the reference in this datasheet.

      ab69778 has been referenced in 2 publications.

      • Shen Y  et al. The immune mechanism of Mycoplasma hyopneumoniae 168 vaccine strain through dendritic cells. BMC Vet Res 13:285 (2017). PubMed: 28915878
      • Campioni D  et al. Immunophenotypic heterogeneity of bone marrow-derived mesenchymal stromal cells from patients with hematologic disorders: correlation with bone marrow microenvironment. Haematologica 91:364-8 (2006). PubMed: 16531259

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Flow Cytometry abreview for Anti-CD80 antibody [MEM-233] (Phycoerythrin), prediluted

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Fixation
      Formaldehyde
      Permeabilization
      No
      Sample
      Human Cell (PBMC (monocyte depleted))
      Specification
      PBMC (monocyte depleted)
      Gating Strategy
      lymphocytes
      Preparation
      Cell harvesting/tissue preparation method: Ficoll
      Sample buffer: DPBS
      Read More

      Abcam user community

      Verified customer

      Submitted Jun 20 2014

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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