PE Anti-CD81 antibody [Eat 2] (ab23553)

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Flow Cytometry - PE Anti-CD81 antibody [Eat 2] (ab23553)

    Key features and details

    • PE Armenian hamster monoclonal [Eat 2] to CD81
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG

    Overview

    • Product name

      PE Anti-CD81 antibody [Eat 2]
      See all CD81 primary antibodies

    • Description

      PE Armenian hamster monoclonal [Eat 2] to CD81

    • Host species

      Armenian hamster

    • Conjugation

      PE. Ex: 488nm, Em: 575nm

    • Specificity

      ab23553 recognises CD81, a 26kD cell surface glycoprotein that is also known as TAPA-1.

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse

    • Immunogen

      Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

    • Positive control

      • Flow Cyt: Mouse blood

    • General notes

      Myeloma: Spleen cells from immunised Armenian hamsters were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line.

      Purified IgG conjugated to R. Phycoerythrin (RPE).

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    Properties

    Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab23553 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Notes
    Application notes
    Flow Cyt: 1/neat.
    Use 10µl for 106 cells in 100µl.

    Note: ab23553 induces homotypic aggregation of B cells and inhibits anti Ig and IL4 induced proliferation. ab23553 requires the presence of both extracellular loops of TAPA1 for binding.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    Target

    • Function

      May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV.

    • Tissue specificity

      Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines.

    • Involvement in disease

      Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6) [MIM:613496]; also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.

    • Sequence similarities

      Belongs to the tetraspanin (TM4SF) family.

    • Post-translational
      modifications

      Not glycosylated.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • 26 kDa cell surface protein TAPA 1 antibody
        • 26 kDa cell surface protein TAPA-1 antibody
        • 26 kDa cell surface protein TAPA1 antibody
        • CD 81 antibody
        • CD81 antibody
        • CD81 antigen (target of antiproliferative antibody 1) antibody
        • CD81 antigen antibody
        • CD81 molecule antibody
        • CD81_HUMAN antibody
        • CVID6 antibody
        • S5.7 antibody
        • TAPA 1 antibody
        • TAPA1 antibody
        • Target of the antiproliferative antibody 1 antibody
        • Tetraspanin 28 antibody
        • Tetraspanin-28 antibody
        • Tetraspanin28 antibody
        • Tspan 28 antibody
        • Tspan-28 antibody
        • Tspan28 antibody
        see all

      Images

      • Flow Cytometry - PE Anti-CD81 antibody [Eat 2] (ab23553)
        Flow Cytometry - PE Anti-CD81 antibody [Eat 2] (ab23553)

        Flow cytometric analysis of mouse blood labelling CD81 with ab23553 at 1/10 dilution (Right). Compared with Alexa Fluor® 700 conjugated hamster IgG1 isotype control (Left). Gated on live, single lymphocytes in the presence of 10% mouse serum. Cells were stained with Alexa Fluor 700 conjugated Rat anti Mouse CD19. Data acquired on the ZE5™ Cell Analyzer.

      References (0)

      Publishing research using ab23553? Please let us know so that we can cite the reference in this datasheet.

      ab23553 has not yet been referenced specifically in any publications.

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