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  1. Link

    pe-cd90--thy1-antibody-mrc-ox-7-ab33694.pdf

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Immunology Adaptive Immunity T Cells CD
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PE Anti-CD90 / Thy1 antibody [MRC OX-7] (ab33694)

  • Datasheet
Submit a review Q&A (1)References (10)

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Key features and details

  • PE Mouse monoclonal [MRC OX-7] to CD90 / Thy1
  • Suitable for: Flow Cyt
  • Reacts with: Mouse, Rat
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Isotype: IgG1

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Carrier Free Unconjugated

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Recombinant Human CD90 / Thy1 protein (Fc Chimera) (ab157072)
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Overview

  • Product name

    PE Anti-CD90 / Thy1 antibody [MRC OX-7]
    See all CD90 / Thy1 primary antibodies
  • Description

    PE Mouse monoclonal [MRC OX-7] to CD90 / Thy1
  • Host species

    Mouse
  • Conjugation

    PE. Ex: 488nm, Em: 575nm
  • Specificity

    Since CD90 is a monomorphic determinant in rat but polymorphic in mice, clone MRC OX-7 reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. The affinity of the Fab' of MRC OX-7 for rat Thy1 is 3 x 109m-1 and for mouse Thy1.1 is 3 x 108m-1
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Full length protein corresponding to CD90/ Thy1. Rat CD90 antigen.

  • Positive control

    • Rat and mouse CD90 is expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (mouse), immature B cells (rat) and connective tissues.
  • General notes

    ab33694 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic rat neurons, using fluorescence microscopy.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Primary antibody notes

    ab33694 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic rat neurons, using fluorescence microscopy.
  • Clonality

    Monoclonal
  • Clone number

    MRC OX-7
  • Myeloma

    NS1
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage

Associated products

  • Alternative Versions

    • HRP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab199219)
    • PerCP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab216354)
    • Anti-CD90 / Thy1 antibody [MRC OX-7] - BSA and Azide free (ab222781)
    • Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
    • FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (ab226)
  • Isotype control

    • PE Mouse IgG1 [B11/6] - Isotype Control (ab91357)
  • Recombinant Protein

    • Recombinant Human CD90 / Thy1 protein (Fc Chimera) (ab157072)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab33694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.

or 100ul whole blood. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Notes
Flow Cyt
Use at an assay dependent concentration.

or 100ul whole blood. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P04216 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 21838 Mouse
    • Entrez Gene: 24832 Rat
    • SwissProt: P01831 Mouse
    • SwissProt: P01830 Rat
    • Unigene: 3951 Mouse
    • Unigene: 108198 Rat
    • Alternative names

      • CD7 antibody
      • CD90 antibody
      • CD90 antigen antibody
      • CDw90 antibody
      • FLJ33325 antibody
      • MGC128895 antibody
      • T25 antibody
      • Theta antigen antibody
      • Thy 1 antibody
      • Thy 1 cell surface antigen antibody
      • Thy 1 membrane glycoprotein antibody
      • Thy 1 T cell antigen antibody
      • Thy 1.2 antibody
      • Thy-1 antigen antibody
      • Thy-1 membrane glycoprotein antibody
      • Thy1 antibody
      • Thy1 antigen antibody
      • Thy1 T cell antigen antibody
      • Thy1.1 antibody
      • Thy1.2 antibody
      • THY1_HUMAN antibody
      • Thymus cell antigen 1, theta antibody
      see all

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (10)

    Publishing research using ab33694? Please let us know so that we can cite the reference in this datasheet.

    ab33694 has been referenced in 10 publications.

    • Oh J  et al. Mesenchymal stem cells genetically engineered to express platelet-derived growth factor and heme oxygenase-1 ameliorate osteoarthritis in a canine model. J Orthop Surg Res 16:43 (2021). PubMed: 33430899
    • Zhao B  et al. Transplantation of bone marrow-derived mesenchymal stem cells with silencing of microRNA-138 relieves pelvic organ prolapse through the FBLN5/IL-1ß/elastin pathway. Aging (Albany NY) 13:3045-3059 (2021). PubMed: 33460398
    • Lin Y  et al. In vitro behavior of tendon stem/progenitor cells on bioactive electrospun nanofiber membranes for tendon-bone tissue engineering applications. Int J Nanomedicine 14:5831-5848 (2019). PubMed: 31534327
    • Lam PK  et al. Topical Application of Mesenchymal Stromal Cells Ameliorated Liver Parenchyma Damage After Ischemia-Reperfusion Injury in an Animal Model. Transplant Direct 3:e160 (2017). Flow Cyt ; Rat . PubMed: 28620644
    • Sun W  et al. Proanthocyanidins Attenuation of H2O2-Induced Oxidative Damage in Tendon-Derived Stem Cells via Upregulating Nrf-2 Signaling Pathway. Biomed Res Int 2017:7529104 (2017). PubMed: 29201913
    • Liu X  et al. Mechanical Tension Promotes the Osteogenic Differentiation of Rat Tendon-derived Stem Cells Through the Wnt5a/Wnt5b/JNK Signaling Pathway. Cell Physiol Biochem 36:517-30 (2015). PubMed: 25966835
    • Zhang J  et al. Adenovirus-mediated delivery of the human IFN-? gene potentiates the cytotoxicity of daunorubicin against leukemic cells through downregulation of the a4ß1 integrin/ILK/apoptosis pathway. Oncol Lett 7:361-368 (2014). Flow Cyt ; Human . PubMed: 24396448
    • Rui YF  et al. Altered fate of tendon-derived stem cells isolated from a failed tendon-healing animal model of tendinopathy. Stem Cells Dev 22:1076-85 (2013). Flow Cyt ; Rat . PubMed: 23106341
    • Das A  et al. The promotion of mandibular defect healing by the targeting of S1P receptors and the recruitment of alternatively activated macrophages. Biomaterials 34:9853-62 (2013). PubMed: 24064148
    • Tan Q  et al. Effect of In Vitro Passaging on the Stem Cell-Related Properties of Tendon-Derived Stem Cells-Implications in Tissue Engineering. Stem Cells Dev : (2011). Flow Cyt ; Rat . PubMed: 21627568

    Customer reviews and Q&As

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    Submit a review Submit a question

    Question

    What is concentration after reconstitution?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 27 2011

    Answer

    Thank you for your phone call last week and for your patience while I have been in contact with the lab that produces ab33694. Because of the protein PE conjugate, an exact concentration of IgG can not be measured. An estimated concentration for this product after reconstitution is ~0.1 mg/ml. Please let me know if you have any further questions or concerns and I will be happy to help you.  

    Read More

    Abcam Scientific Support

    Answered on Jun 27 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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