Recombinant PE Anti-Choline Acetyltransferase antibody [EPR13024(B)] (ab224000)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR13024(B)] to Choline Acetyltransferase
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
Overview
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Product name
PE Anti-Choline Acetyltransferase antibody [EPR13024(B)]
See all Choline Acetyltransferase primary antibodies -
Description
PE Rabbit monoclonal [EPR13024(B)] to Choline Acetyltransferase -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (intra): SHSY5Y cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13024(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Choline Acetyltransferase antibody [EPR13024(B)] (ab181023)
- Alexa Fluor® 488 Anti-Choline Acetyltransferase antibody [EPR13024(B)] (ab192465)
- Alexa Fluor® 647 Anti-Choline Acetyltransferase antibody [EPR13024(B)] (ab192466)
- APC Anti-Choline Acetyltransferase antibody [EPR13024(B)] (ab224001)
- Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)
- Alexa Fluor® 647 Anti-Choline Acetyltransferase antibody [EPR16590] (ab225262)
- Alexa Fluor® 488 Anti-Choline Acetyltransferase antibody [EPR16590] (ab225263)
- Alexa Fluor® 568 Anti-Choline Acetyltransferase antibody [EPR13024(B)] (ab302805)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224000 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/5000.
The cellular localisation of this product has been verified in ICC/IF. |
Notes |
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Flow Cyt (Intra)
1/5000. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. -
Involvement in disease
Myasthenic syndrome, congenital, 6, presynaptic -
Sequence similarities
Belongs to the carnitine/choline acetyltransferase family. - Information by UniProt
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Database links
- Entrez Gene: 1103 Human
- Entrez Gene: 12647 Mouse
- Entrez Gene: 290567 Rat
- Omim: 118490 Human
- SwissProt: P28329 Human
- SwissProt: Q03059 Mouse
- SwissProt: P32738 Rat
- Unigene: 302002 Human
see all -
Alternative names
- Acetyl CoA choline O acetyltransferase antibody
- Acetyl CoA:choline O acetyltransferase antibody
- ChAT antibody
see all
Images
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Overlay histogram showing SHSY5Y cells stained with ab224000 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab224000, 1/5000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab224000 has not yet been referenced specifically in any publications.