Recombinant PE Anti-Cortactin antibody [EP1922Y] (ab209758)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EP1922Y] to Cortactin
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
Overview
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Product name
PE Anti-Cortactin antibody [EP1922Y]
See all Cortactin primary antibodies -
Description
PE Rabbit monoclonal [EP1922Y] to Cortactin -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (intra): HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1922Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-Cortactin antibody [EP1922Y] (ab202648)
- Alexa Fluor® 647 Anti-Cortactin antibody [EP1922Y] (ab202650)
- Alexa Fluor® 555 Anti-Cortactin antibody [EP1922Y] (ab215298)
- Alexa Fluor® 594 Anti-Cortactin antibody [EP1922Y] (ab215300)
- Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)
- Anti-Cortactin antibody [EP1922Y] (ab81208)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab209758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/5000.
|
Notes |
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Flow Cyt (Intra)
1/5000. |
Target
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Function
Contributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases. -
Sequence similarities
Contains 7 cortactin repeats.
Contains 1 SH3 domain. -
Domain
The SH3 motif may mediate binding to the cytoskeleton. -
Post-translational
modificationsTyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation. -
Cellular localization
Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle. Associated with membrane ruffles and lamellipodia. - Information by UniProt
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Database links
- Entrez Gene: 2017 Human
- Entrez Gene: 13043 Mouse
- Entrez Gene: 60465 Rat
- GenBank: NM_138565 Human
- GenBank: NM_007803 Mouse
- Omim: 164765 Human
- SwissProt: Q14247 Human
- SwissProt: Q60598 Mouse
see all -
Alternative names
- Amplaxin antibody
- CTTN antibody
- EMS 1 antibody
see all
Images
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Overlay histogram showing HeLa cells stained with ab209758 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209758, 1/5000 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab209758 has not yet been referenced specifically in any publications.