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RecombinantRabMAb

Recombinant PE Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab209581)

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  • Certificate of Compliance
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Flow Cytometry - PE Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab209581)
  • PE Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab209581)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [EPR5866] to COX1 / Cyclooxygenase 1
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

You may also be interested in

Protein
Product image
Recombinant human COX1 / Cyclooxygenase 1 protein (ab198643)

View more associated products

Overview

  • Product name

    PE Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866]
    See all COX1 / Cyclooxygenase 1 primary antibodies
  • Description

    PE Rabbit monoclonal [EPR5866] to COX1 / Cyclooxygenase 1
  • Host species

    Rabbit
  • Conjugation

    PE. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human COX1/ Cyclooxygenase 1 aa 100-200. The exact sequence is proprietary.
    Database link: P23219

  • Positive control

    • Flow Cyt: HeLa cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5866
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cardiovascular
    • Blood
    • Platelets
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Prostaglandins
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
    • Alexa Fluor® 488 Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199027)
    • Alexa Fluor® 647 Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199202)
    • HRP Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab199203)
    • Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] - BSA and Azide free (ab219375)
  • Isotype control

    • PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
  • Recombinant Protein

    • Recombinant human COX1 / Cyclooxygenase 1 protein (ab198643)

Applications

Our Abpromise guarantee covers the use of ab209581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.

Target

  • Function

    May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.
  • Pathway

    Lipid metabolism; prostaglandin biosynthesis.
  • Sequence similarities

    Belongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • Cellular localization

    Microsome membrane. Endoplasmic reticulum membrane.
  • Target information above from: UniProt accession P23219 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5742 Human
    • Entrez Gene: 19224 Mouse
    • Entrez Gene: 24693 Rat
    • Omim: 176805 Human
    • SwissProt: P23219 Human
    • SwissProt: P22437 Mouse
    • SwissProt: Q63921 Rat
    • Unigene: 201978 Human
    • Unigene: 275434 Mouse
    • Unigene: 44404 Rat
    see all
  • Alternative names

    • COX 1 antibody
    • COX 3 antibody
    • COX-1 antibody
    • COX1 antibody
    • Cox3 antibody
    • Cyclooxygenase 1 antibody
    • Cyclooxygenase 3, included antibody
    • Cyclooxygenase-1 antibody
    • EC 1.14.99.1 antibody
    • Partial COX1 proteins, included antibody
    • PCOX1 antibody
    • PGG/HS antibody
    • PGH synthase 1 antibody
    • PGH1_HUMAN antibody
    • PGHS 1 antibody
    • PGHS-1 antibody
    • PGHS1 antibody
    • PHS 1 antibody
    • PHS1 antibody
    • Prostaglandin endoperoxide synthase 1 antibody
    • Prostaglandin G/H synthase 1 antibody
    • Prostaglandin H2 synthase 1 antibody
    • Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin-endoperoxide synthase 1 antibody
    • PTGHS antibody
    • PTGS1 antibody
    see all

Images

  • Flow Cytometry - PE Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab209581)
    Flow Cytometry - PE Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab209581)

    Overlay histogram showing HeLa cells stained with ab209581 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol for 30 min at -20°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209581, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 585/40 bandpass filter.

  • PE Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab209581)
    PE Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab209581)

Protocols

  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab209581? Please let us know so that we can cite the reference in this datasheet.

    ab209581 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab209581.
    Please use the links above to contact us or submit feedback about this product.

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