Recombinant PE Anti-CRISPR-Cas9 antibody [EPR19799] (ab215701)
![Flow Cytometry - PE Anti-CRISPR-Cas9 antibody [EPR19799] (ab215701)](https://www.abcam.com/ps/products/215/ab215701/Images/ab215701-312609-anti-crispr-cas9-antibody-epr19799-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-CRISPR-Cas9 antibody [EPR19799] (ab215701)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR19799] to CRISPR-Cas9
- Suitable for: Flow Cyt
- Reacts with: Staphylococcus aureus
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
PE Anti-CRISPR-Cas9 antibody [EPR19799]
See all CRISPR-Cas9 primary antibodies -
Description
PE Rabbit monoclonal [EPR19799] to CRISPR-Cas9 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Staphylococcus aureus -
Immunogen
Synthetic peptide within CRISPR-Cas9 aa 100-200. The exact sequence is proprietary. Staphylococcus aureus
Database link: J7RUA5 -
Positive control
- Flow Cyt: 293T cells transfected with CRISPR-Cas9 with Myc tag
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19799 -
Isotype
IgG
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab215701 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/500. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Relevance
[FUNCTION] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself. -
Database links
- SwissProt: J7RUA5 Staphylococcus aureus
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Alternative names
- Cas9 antibody
- CRISPR-associated endonuclease Cas9/Csn1 antibody
- CRISPR-Cas9/Csn1 antibody
see all
Images
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Flow Cytometry - PE Anti-CRISPR-Cas9 antibody [EPR19799] (ab215701)
Flow cytometric analysis of 293T transfected with CRISPR-Cas9 with Myc tag stained with ab215701 (right panel). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab215701, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (left panel) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Co-staining with anti-myc (y-axis).
Acquisition of >5,000 events were collected using a 20 mW blue solid-state laser (488nm) and 585/42 bandpass filter.
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![PE Anti-CRISPR-Cas9 antibody [EPR19799] (ab215701)](https://www.abcam.com/ps/products/215/ab215701/Images/ab215701-3-benefits-of-recombinant-antibodies.png)
PE Anti-CRISPR-Cas9 antibody [EPR19799] (ab215701)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab215701 has not yet been referenced specifically in any publications.
