Recombinant PE Anti-Cyclin B1 antibody [Y106] (ab210615)
![Flow Cytometry - PE Anti-Cyclin B1 antibody [Y106] (ab210615)](https://www.abcam.com/ps/products/210/ab210615/Images/ab210615-261619-anti-cyclin-b1-antibody-y106-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-Cyclin B1 antibody [Y106] (ab210615)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [Y106] to Cyclin B1
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
PE Anti-Cyclin B1 antibody [Y106]
See all Cyclin B1 primary antibodies -
Description
PE Rabbit monoclonal [Y106] to Cyclin B1 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
This antibody does not cross-react with other cyclin family members. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Cyclin B1 aa 400 to the C-terminus. The exact sequence is proprietary.
Database link: P14635 -
Positive control
- Flow Cyt: HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y106 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab210615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/500. The cellular localisation of this product has been verified in ICC/IF |
Target
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Function
Essential for the control of the cell cycle at the G2/M (mitosis) transition. -
Sequence similarities
Belongs to the cyclin family. Cyclin AB subfamily. -
Developmental stage
Accumulates steadily during G2 and is abruptly destroyed at mitosis. -
Post-translational
modificationsUbiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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Database links
- Entrez Gene: 891 Human
- Omim: 123836 Human
- SwissProt: P14635 Human
- Unigene: 23960 Human
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Alternative names
- CCNB 1 antibody
- CCNB antibody
- ccnb1 antibody
see all
Images
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Flow Cytometry - PE Anti-Cyclin B1 antibody [Y106] (ab210615)
Overlay histogram showing HeLa cells stained with ab210615 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol for 30 min at -20°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210615, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
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![PE Anti-Cyclin B1 antibody [Y106] (ab210615)](https://www.abcam.com/ps/products/210/ab210615/Images/ab210615-3-benefits-of-recombinant-antibodies.png)
PE Anti-Cyclin B1 antibody [Y106] (ab210615)
Datasheets and documents
Certificate of Compliance
References (0)
ab210615 has not yet been referenced specifically in any publications.
