Recombinant PE Anti-Cytokeratin 20 antibody [EPR1622Y] (ab209923)
![Flow Cytometry - PE Anti-Cytokeratin 20 antibody [EPR1622Y] (ab209923)](https://www.abcam.com/ps/products/209/ab209923/Images/ab209923-267969-anti-cytokeratin-20-antibody-epr1622y-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-Cytokeratin 20 antibody [EPR1622Y] (ab209923)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR1622Y] to Cytokeratin 20
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
PE Anti-Cytokeratin 20 antibody [EPR1622Y]
See all Cytokeratin 20 primary antibodies -
Description
PE Rabbit monoclonal [EPR1622Y] to Cytokeratin 20 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Goat, Pig, Common marmoset
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Immunogen
Synthetic peptide within Human Cytokeratin 20 aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P35900 -
Positive control
- Flow Cyt: JEG3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1622Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab209923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/500. |
Target
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Function
Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine. -
Tissue specificity
Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa. -
Sequence similarities
Belongs to the intermediate filament family. -
Developmental stage
First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative. -
Post-translational
modificationsHyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury.
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 54474 Human
- Entrez Gene: 286912 Rat
- Omim: 608218 Human
- SwissProt: P35900 Human
- SwissProt: P25030 Rat
- Unigene: 84905 Human
- Unigene: 9887 Rat
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Alternative names
- CD20 antibody
- CK 20 antibody
- CK-20 antibody
see all
Images
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Flow Cytometry - PE Anti-Cytokeratin 20 antibody [EPR1622Y] (ab209923)
Overlay histogram showing JEG3 cells stained with ab209923 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209923, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
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![PE Anti-Cytokeratin 20 antibody [EPR1622Y] (ab209923)](https://www.abcam.com/ps/products/209/ab209923/Images/ab209923-2-benefits-of-recombinant-antibodies.png)
PE Anti-Cytokeratin 20 antibody [EPR1622Y] (ab209923)
Datasheets and documents
Certificate of Compliance
References (0)
ab209923 has not yet been referenced specifically in any publications.
