Recombinant PE Anti-Cytokeratin 5 antibody [EP1601Y] (ab224985)
![Flow Cytometry - PE Anti-Cytokeratin 5 antibody [EP1601Y] (ab224985)](https://www.abcam.com/ps/products/224/ab224985/Images/ab224985-312650-anti-cytokeratin-5-antibody-ep1601y-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-Cytokeratin 5 antibody [EP1601Y] (ab224985)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EP1601Y] to Cytokeratin 5
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
PE Anti-Cytokeratin 5 antibody [EP1601Y]
See all Cytokeratin 5 primary antibodies -
Description
PE Rabbit monoclonal [EP1601Y] to Cytokeratin 5 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human Cytokeratin 5 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P13647 -
Positive control
- Flow Cyt: A431 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EP1601Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-Cytokeratin 5 antibody [EP1601Y] (ab193894)
- Alexa Fluor® 647 Anti-Cytokeratin 5 antibody [EP1601Y] (ab193895)
- HRP Anti-Cytokeratin 5 antibody [EP1601Y] (ab193896)
- Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (ab214586)
- APC Anti-Cytokeratin 5 antibody [EP1601Y] (ab224984)
- Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (ab52635)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab224985 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/5000. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Involvement in disease
Defects in KRT5 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
Defects in KRT5 are the cause of epidermolysis bullosa simplex with migratory circinate erythema (EBSMCE) [MIM:609352]. EBSMCE is a form of intraepidermal epidermolysis bullosa characterized by unusual migratory circinate erythema. Skin lesions appear from birth primarily on the hands, feet, and legs but spare nails, ocular epithelia and mucosae. Lesions heal with brown pigmentation but no scarring. Electron microscopy findings are distinct from those seen in the DM-EBS, with no evidence of tonofilament clumping.
Defects in KRT5 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.
Defects in KRT5 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, althought it is less severe.
Defects in KRT5 are the cause of epidermolysis bullosa simplex with mottled pigmentation (MP-EBS) [MIM:131960]. MP-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering at acral sites and 'mottled' pigmentation of the trunk and proximal extremities with hyper- and hypopigmentation macules.
Defects in KRT5 are the cause of Dowling-Degos disease (DDD) [MIM:179850]; also known as Dowling-Degos-Kitamura disease or reticulate acropigmentation of Kitamura. DDD is an autosomal dominant genodermatosis. Affected individuals develop a postpubertal reticulate hyperpigmentation that is progressive and disfiguring, and small hyperkeratotic dark brown papules that affect mainly the flexures and great skin folds. Patients usually show no abnormalities of the hair or nails. -
Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
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Database links
- Entrez Gene: 3852 Human
- Entrez Gene: 110308 Mouse
- Omim: 148040 Human
- SwissProt: P13647 Human
- SwissProt: Q922U2 Mouse
- Unigene: 433845 Human
- Unigene: 451847 Mouse
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Alternative names
- 58 kDa cytokeratin antibody
- CK-5 antibody
- CK5 antibody
see all
Images
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Flow Cytometry - PE Anti-Cytokeratin 5 antibody [EP1601Y] (ab224985)
Overlay histogram showing A431 cells stained with ab224985 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab224985, 1/5000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in A431 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
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![PE Anti-Cytokeratin 5 antibody [EP1601Y] (ab224985)](https://www.abcam.com/ps/products/224/ab224985/Images/ab224985-3-benefits-of-recombinant-antibodies.png)
PE Anti-Cytokeratin 5 antibody [EP1601Y] (ab224985)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab224985 has not yet been referenced specifically in any publications.
