RecombinantRabMAb

Recombinant PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab210628)

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Immunocytochemistry/ Immunofluorescence - PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab210628)
  • Flow Cytometry - PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab210628)
  • PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab210628)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [EPR1619Y] to Cytokeratin 7 - Cytoskeleton Marker
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Overview

  • Product name

    PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker
    See all Cytokeratin 7 primary antibodies

  • Description

    PE Rabbit monoclonal [EPR1619Y] to Cytokeratin 7 - Cytoskeleton Marker

  • Host species

    Rabbit

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Cytokeratin 7 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P08729

  • Positive control

    • Flow Cytometry: T47D cells ICC/IF: T47D cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab210628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in T47D cells fixed with 100% methanol (5 min), and 4% formaldehyde (10 min)
Flow Cyt 1/500.

Target

  • Function

    Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

  • Tissue specificity

    Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.

  • Sequence similarities

    Belongs to the intermediate filament family.

  • Post-translational
    modifications

    Arg-20 is dimethylated, probably to asymmetric dimethylarginine.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • CK 7 antibody
      • CK-7 antibody
      • CK7 antibody
      • Cytokeratin 7 antibody
      • Cytokeratin-7 antibody
      • D15Wsu77e antibody
      • K2C7 antibody
      • K2C7_HUMAN antibody
      • K7 antibody
      • Keratin 7 antibody
      • Keratin 7, type II antibody
      • Keratin type II cytoskeletal 7 antibody
      • Keratin, 55K type II cytoskeletal antibody
      • Keratin, simple epithelial antibody
      • Keratin, simple epithelial type I, K7 antibody
      • Keratin, type II cytoskeletal 7 antibody
      • Keratin-7 antibody
      • Krt2-7 antibody
      • KRT7 antibody
      • MGC11625 antibody
      • MGC129731 antibody
      • MGC3625 antibody
      • Sarcolectin antibody
      • SCL antibody
      • Type II mesothelial keratin K7 antibody
      • Type-II keratin Kb7 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab210628)
      Immunocytochemistry/ Immunofluorescence - PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab210628)

      Figure Legend for the image: ab210628 staining Cytokeratin 7 in T74D cells. The cells were fixed with 100%methanol, permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab210628 at 1/100 dilution (pseudocolored in green). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      This product also gave a positive signal under the same testing conditions in T74D cells fixed with 4%formaldehyde.

    • Flow Cytometry - PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab210628)
      Flow Cytometry - PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab210628)

      Overlay histogram showing T47D cells stained with ab210628 (red line). The cells were fixed with 4% formaldehyde and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210628, 1/500 dilution) for 30 min at 22°C.

      Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    • PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab210628)
      PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab210628)

    References (0)

    Publishing research using ab210628? Please let us know so that we can cite the reference in this datasheet.

    ab210628 has not yet been referenced specifically in any publications.

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