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  1. Link

    pe-ddddk-tag-binds-to-flag-tag-sequence-antibody-m2-ab72469.pdf

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Tags & Cell Markers Epitope Tags DDDDK Tag
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PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (4)

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Flow Cytometry - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469)
  • Sandwich ELISA - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469)

Key features and details

  • PE Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence)
  • Suitable for: Flow Cyt, Sandwich ELISA
  • Reacts with: Species independent
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Isotype: IgG1

Overview

  • Product name

    PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]
    See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies
  • Description

    PE Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence)
  • Host species

    Mouse
  • Conjugation

    PE. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cyt, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence).

  • General notes

    Excitation max. lambda: 565>498 nm Emission max. lambda: 578 nm F/P 1:1

    FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.013% Sodium azide
    Constituents: 0.1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate, 1.71% Sucrose
  • Concentration information loading...
  • Purity

    Size exclusion
  • Purification notes

    Size exclusion chromatography as well as proprietary methods for purifying the final materials were used.
  • Clonality

    Monoclonal
  • Clone number

    M2
  • Isotype

    IgG1
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • DDDDK Tag

Associated products

  • Alternative Versions

    • DyLight® 650 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117492)
    • DyLight® 550 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117495)
    • DyLight® 488 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117505)
    • PerCP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117514)
    • Europium Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab187914)
    • HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
    • SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72569)
    • Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab81655)
  • Isotype control

    • PE Mouse IgG1 [B11/6] - Isotype Control (ab91357)

Applications

Our Abpromise guarantee covers the use of ab72469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Sandwich ELISA Use at an assay dependent concentration.

Target

  • Relevance

    This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence).
  • Alternative names

    • DDDDK epitope tag antibody
    • DDDK antibody
    • ddk antibody
    • DYKDDDDK (also known as the FLAG® tag sequence) antibody
    • DYKDDDDK antibody
    • DYKDDDDK epitope tag antibody
    • DYKDDDDK tag antibody
    • ECS epitope tag antibody
    • ECS tag antibody
    • Enterokinase Cleavage Site epitope tag antibody
    • Enterokinase Cleavage Site tag antibody
    • FLAG® antibody
    • FLAG® tag antibody
    see all

Images

  • Flow Cytometry - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469)
    Flow Cytometry - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469)

    Flow cytometry analysis of a recombinant (N-terminus DDDDK-tag) transmembrane cell surface receptor in CHO cells using ab72469 (blue). Black - Mouse IgG1 Phycoerythrin isotype control.

  • Sandwich ELISA - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469)
    Sandwich ELISA - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469)
    Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated DYKDDDDK tagged peptide and read after a 2 hour incubation at room temperature with ab72469.

Protocols

  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (4)

    Publishing research using ab72469? Please let us know so that we can cite the reference in this datasheet.

    ab72469 has been referenced in 4 publications.

    • Sharma S  et al. Genome-scale identification of cellular pathways required for cell surface recognition. Genome Res 28:1372-1382 (2018). PubMed: 29914970
    • AlHajri SM  et al. Ovine Herpesvirus 2 Glycoproteins B, H, and L Are Sufficient for, and Viral Glycoprotein Ov8 Can Enhance, Cell-Cell Membrane Fusion. J Virol 91:N/A (2017). Flow Cyt . PubMed: 28053110
    • Compton AA  et al. Natural mutations in IFITM3 modulate post-translational regulation and toggle antiviral specificity. EMBO Rep 17:1657-1671 (2016). PubMed: 27601221
    • Raphel L  et al. Myocardin overexpression is sufficient for promoting the development of a mature smooth muscle cell-like phenotype from human embryonic stem cells. PLoS One 7:e44052 (2012). Flow Cyt . PubMed: 22937150

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    We would like to find out the average number of PE molecules per antibody for the following product:
    Anti-DDDDK tag antibody [M2] (Phycoerythrin) (ab72469)


    Read More

    Abcam community

    Verified customer

    Asked on Aug 27 2014

    Answer


    Data from recent batches indicates that there is approximately a 1:1 ration of PE to each antibody molecule.

    Read More

    Sam Washer

    Abcam Scientific Support

    Answered on Aug 27 2014

    Western blot abreview for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (Phycoerythrin)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293 cells transfected with empty vector or FH-FLAG)
    Gel Running Conditions
    Reduced Denaturing (Pierce precise protein gel 4-20%)
    Loading amount
    20 µg
    Specification
    293 cells transfected with empty vector or FH-FLAG
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Mr. Antibody Solutions

    Verified customer

    Submitted Dec 17 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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