PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469)
![Flow Cytometry - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469)](https://www.abcam.com/ps/products/72/ab72469/Images/ab72469-241230-anti-ddddk-tag-antibody-m2-equivalent-to-flag-antibodies-from-sigma-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469)")
Key features and details
- PE Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: Flow Cyt, Sandwich ELISA
- Reacts with: Species independent
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]
See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies -
Description
PE Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence) -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt, Sandwich ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence).
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General notes
Excitation max. lambda: 565>498 nm Emission max. lambda: 578 nm F/P 1:1
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.013% Sodium azide
Constituents: 0.1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate, 1.71% Sucrose -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
Size exclusion chromatography as well as proprietary methods for purifying the final materials were used. -
Clonality
Monoclonal -
Clone number
M2 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- DyLight® 650 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117492)
- DyLight® 550 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117495)
- DyLight® 488 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117505)
- PerCP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab117514)
- Europium Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab187914)
- HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
- SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72569)
- Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab81655)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Sandwich ELISA
Use at an assay dependent concentration. |
Target
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Relevance
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
Alternative names
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
Images
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Flow Cytometry - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469)
Flow cytometry analysis of a recombinant (N-terminus DDDDK-tag) transmembrane cell surface receptor in CHO cells using ab72469 (blue). Black - Mouse IgG1 Phycoerythrin isotype control.
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![Sandwich ELISA - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469)](https://www.abcam.com/ps/products/72/ab72469/Images/ab72469-87664-anti-ddddk-tag-antibody-m2-equivalent-to-flag-antibodies-from-sigma-phycoerythrin-sandwich-elisa.jpg)
Sandwich ELISA - PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72469)Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated DYKDDDDK tagged peptide and read after a 2 hour incubation at room temperature with ab72469.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab72469 has been referenced in 6 publications.
- Edelstein HI et al. Elucidation and refinement of synthetic receptor mechanisms. Synth Biol (Oxf) 5:ysaa017 (2020). PubMed: 33392392
- Sharma S et al. Genome-scale identification of cellular pathways required for cell surface recognition. Genome Res 28:1372-1382 (2018). PubMed: 29914970
- AlHajri SM et al. Ovine Herpesvirus 2 Glycoproteins B, H, and L Are Sufficient for, and Viral Glycoprotein Ov8 Can Enhance, Cell-Cell Membrane Fusion. J Virol 91:N/A (2017). Flow Cyt . PubMed: 28053110
- Leshchiner ES et al. Small-molecule inhibitors directly target CARD9 and mimic its protective variant in inflammatory bowel disease. Proc Natl Acad Sci U S A 114:11392-11397 (2017). PubMed: 29073062
- Compton AA et al. Natural mutations in IFITM3 modulate post-translational regulation and toggle antiviral specificity. EMBO Rep 17:1657-1671 (2016). PubMed: 27601221
- Raphel L et al. Myocardin overexpression is sufficient for promoting the development of a mature smooth muscle cell-like phenotype from human embryonic stem cells. PLoS One 7:e44052 (2012). Flow Cyt . PubMed: 22937150