Recombinant Anti-DDX39 antibody [EPR13507(B)] (PE) (ab225253)
![Flow Cytometry - Anti-DDX39 antibody [EPR13507(B)] (Phycoerythrin) (ab225253)](https://www.abcam.com/ps/products/225/ab225253/Images/ab225253-317223-anti-ddx39-antibody-epr13507b-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - Anti-DDX39 antibody [EPR13507(B)] (Phycoerythrin) (ab225253)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR13507(B)] to DDX39
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
Anti-DDX39 antibody [EPR13507(B)] (PE)
See all DDX39 primary antibodies -
Description
Rabbit monoclonal [EPR13507(B)] to DDX39 (PE) -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human DDX39 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: O00148 -
Positive control
- Flow Cyt: HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13507(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab225253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/5000. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Relevance
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX39 encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants encoding different isoforms. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 10212 Human
- Entrez Gene: 68278 Mouse
- Entrez Gene: 89827 Rat
- SwissProt: O00148 Human
- SwissProt: Q8VDW0 Mouse
- SwissProt: Q5U216 Rat
- Unigene: 311609 Human
- Unigene: 28222 Mouse
see all -
Alternative names
- ATP dependent RNA helicase DDX39 antibody
- ATP-dependent RNA helicase DDX39A antibody
- BAT1 antibody
see all
Images
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Flow Cytometry - Anti-DDX39 antibody [EPR13507(B)] (Phycoerythrin) (ab225253)
Overlay histogram showing HeLa cells stained with ab225253 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225253, 1/5000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
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![Anti-DDX39 antibody [EPR13507(B)] (PE) (ab225253)](https://www.abcam.com/ps/products/225/ab225253/Images/ab225253-3-benefits-of-recombinant-antibodies.png)
Anti-DDX39 antibody [EPR13507(B)] (PE) (ab225253)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab225253 has not yet been referenced specifically in any publications.
