Recombinant PE Anti-Dnmt1 antibody [EPR3522] (ab208999)
![Flow Cytometry - PE Anti-Dnmt1 antibody [EPR3522] (ab208999)](https://www.abcam.com/ps/products/208/ab208999/Images/ab208999-259498-anti-dnmt1-antibody-epr3522-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-Dnmt1 antibody [EPR3522] (ab208999)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR3522] to Dnmt1
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
-
Product name
PE Anti-Dnmt1 antibody [EPR3522]
See all Dnmt1 primary antibodies -
Description
PE Rabbit monoclonal [EPR3522] to Dnmt1 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Dnmt1 aa 1600 to the C-terminus. The exact sequence is proprietary.
Database link: P26358 -
Positive control
- Flow Cyt: HeLa cells ICC/IF: HeLa cells
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3522 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab208999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/250. This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) |
|
| Flow Cyt | 1/2500. |
Target
-
Function
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. -
Tissue specificity
Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1. -
Sequence similarities
Belongs to the C5-methyltransferase family.
Contains 2 BAH domains.
Contains 1 CXXC-type zinc finger. -
Domain
The N-terminal part is required for homodimerization and acts as a regulatory domain. -
Post-translational
modificationsSumoylated; sumoylation increases activity. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 1786 Human
- Omim: 126375 Human
- SwissProt: P26358 Human
- Unigene: 202672 Human
-
Alternative names
- ADCADN antibody
- AIM antibody
- CXXC finger protein 9 antibody
see all
Images
-
Flow Cytometry - PE Anti-Dnmt1 antibody [EPR3522] (ab208999)
Overlay histogram showing HeLa cells stained with ab208999 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab208999, 1/2500 dilution) for 30 min at 22ºC.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50mW Yellow-Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
-
![Immunocytochemistry/ Immunofluorescence - PE Anti-Dnmt1 antibody [EPR3522] (ab208999)](https://www.abcam.com/ps/products/208/ab208999/Images/ab208999-258980-anti-dnmt1-antibody-epr3522-phycoerythrin-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - PE Anti-Dnmt1 antibody [EPR3522] (ab208999)ab208999 staining Dnmt1 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208999 at 1/250 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
-
![PE Anti-Dnmt1 antibody [EPR3522] (ab208999)](https://www.abcam.com/ps/products/208/ab208999/Images/ab208999-3-benefits-of-recombinant-antibodies.png)
PE Anti-Dnmt1 antibody [EPR3522] (ab208999)
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab208999 has not yet been referenced specifically in any publications.
