PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751)
![Flow Cytometry - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751)](https://www.abcam.com/ps/products/90/ab90751/Images/ENPP3-Primary-antibodies-ab90751-1.jpg "Flow Cytometry - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751)")
Key features and details
- PE Mouse monoclonal [NP4D6] to ENPP3/B10
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-ENPP3/B10 antibody [NP4D6]
See all ENPP3/B10 primary antibodies -
Description
PE Mouse monoclonal [NP4D6] to ENPP3/B10 -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human ENPP3/B10.
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General notes
The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions.
This product was previously labelled as ENPP3
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
The unconjugated antibody was purified by protein A (G) affinity chromatography (>95% by SDS-PAGE). After conjugation, the antibody was purified by size-exclusion chromatography and adjusted for direct use. -
Clonality
Monoclonal -
Clone number
NP4D6 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab90751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | ||
| ICC/IF |
ICC/IF: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Cleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD. -
Tissue specificity
Expressed in bile ducts, prostate, uterus and colon. Exclusively expressed on basophils, mast cells and their progenitors. -
Sequence similarities
Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
Contains 2 SMB (somatomedin-B) domains. -
Post-translational
modificationsN-glycosylation is necessary for correct trafficking to the apical surface, but is not the apical targeting signal.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both. -
Cellular localization
Membrane. Secreted. Located to the apical surface in intestinal and kidney epithelial cells. Located to the cell surface of basophils, and to the apical plasma membrane of bile duct cells. Secreted in serum, and in lumen of epithelial cells. - Information by UniProt
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Database links
- Entrez Gene: 5167 Human
- Entrez Gene: 5169 Human
- Omim: 602182 Human
- SwissProt: O14638 Human
- SwissProt: P22413 Human
- Unigene: 486489 Human
- Unigene: 527295 Human
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Alternative names
- Alkaline phosphodiesterase I antibody
- ARHR2 antibody
- B10 antibody
see all
Images
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Flow Cytometry - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751)
ab90751 staining ENPP3/B10 on the surface of human basophils isolated from allergen-stimulated whole blood by flow cytometery.
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![Immunocytochemistry/ Immunofluorescence - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751)](https://www.abcam.com/ps/products/90/ab90751/Images/ENPP3-Primary-antibodies-ab90751-2.jpg)
Immunocytochemistry/ Immunofluorescence - PE Anti-ENPP3/B10 antibody [NP4D6] (ab90751)Surface staining of activated human basophil by FITC-conjugated anti-CD63 (clone MEM-259, ab18235, green) and PE-conjugated anti-ENPP3/B10 (clone NP4D6, ab90751, red); merged signal yellow. DNA visualized by DAPI (blue).
Protocols
References (0)
ab90751 has not yet been referenced specifically in any publications.
