PE Anti-F4/80 antibody [CI:A3-1] (ab105156)
Key features and details
- PE Rat monoclonal [CI:A3-1] to F4/80
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2b
Overview
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Product name
PE Anti-F4/80 antibody [CI:A3-1]
See all F4/80 primary antibodies -
Description
PE Rat monoclonal [CI:A3-1] to F4/80 -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
ab105156 recognises the murine F4/80 antigen. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Thioglycollate stimulated peritoneal cavity macrophages from C57/BL mice.
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Positive control
- Mouse J774 cells
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General notes
Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 5% Sucrose, PBS -
Concentration information loading...
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Purity
Protein A purified -
Primary antibody notes
Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes. -
Clonality
Monoclonal -
Clone number
CI:A3-1 -
Myeloma
NS1 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab105156 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt | Use 10µl for 106 cells. in 100 µl. ab136585 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. -
Tissue specificity
Expression is restricted to eosinophils. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 13733 Mouse
- SwissProt: Q61549 Mouse
- Unigene: 2254 Mouse
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Alternative names
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
see all
Images
Protocols
Datasheets and documents
References (4)
ab105156 has been referenced in 4 publications.
- Shimizu Y et al. A simple culture method for liver and intestinal tissue-resident macrophages from neonatal mice. In Vitro Cell Dev Biol Anim 55:436-444 (2019). PubMed: 31119642
- Li H et al. Fibroblast growth factor 21 increases insulin sensitivity through specific expansion of subcutaneous fat. Nat Commun 9:272 (2018). PubMed: 29348470
- Huang Z et al. The FGF21-CCL11 Axis Mediates Beiging of White Adipose Tissues by Coupling Sympathetic Nervous System to Type 2 Immunity. Cell Metab 26:493-508.e4 (2017). PubMed: 28844880
- Neng L et al. Isolation and culture of endothelial cells, pericytes and perivascular resident macrophage-like melanocytes from the young mouse ear. Nat Protoc 8:709-20 (2013). PubMed: 23493068