PE Anti-F4/80 antibody [CI:A3-1] (ab105156)

Vielen Dank für Ihre Anfrage.

Leider ist es nicht möglich die Konzentration von ab105156 zu bestimmen.

Bei ab105156 handelt es sich einen konjugierten Antikörper.

Die Konzentration von Antikörpern wird über die Proteinmenge bestimmt. ab105156 enthält aber nicht nur den spezifischen Antikörper sondern auch Phycoerythrin und macht deshalb eine Konzentrationsbestimmung nach der Konjugationunmöglich.

Wir empfehlen als Anhaltspunkt eine Konzentration von 0.1 mg/ml anzunehmen.

Es tut mir leid, dass ich Ihnen keine genaueren Angaben machen konnte und hoffe diese Information ist dennoch hilfreich.

Ich wünsche Ihnen viel Erfolg mit Ihrer Forschung und verbleibe

Thank you for your inquiry.

I can confirm that the volume of ab105156 is 500ul per vial. The concentration cannot be determined since this a PE-conjugated antibody. An estimatewould be ˜ 0.1mg/ml.

I suggest to use two concentrations for the first experiment to get results that will be meaningful for further optimization. I recommend 1:50 and and 1:500. Optimal concentrations always have to be determined by the end user.

I am sorry I could not provide a more exact answer to your question on this occasion and hope this information is nevertheless helpful to your customer.

Thank you for your query. I can confirm that the unconjugated clone of ab105156 was typically used at around 10ug/ml for frozen sections. However, I would still recommend to titrate the antibody in order to determine the optimal concentration for your specific experimental requirements. Although we have not specifically measured the concentration of the antibody after conjugation, based on our records, we would expect the concentration of this product to be approximately 0.1mg/ml. 50 test means this antibody can be used for 50 tests in flow cytometry when used at 10µl for 10 x 6 cells (in 100 µl.). I hope this information is helpful. Please do not hesitate to contact me again if you have any further questions.

Thank you for your interest. Just to confirm ab105156 is a primary antibody which is conjugated to PE. This means that it is not necessary to apply a compatible secondary antibody for detection. For further details (tested applications, purification, unit size etc) please take a look at the on-line datasheet: https://www.abcam.com/f4-80-antibody-ci-a3-1-phycoerythrin-ab105156.html If you need any further assistance in the future, please do not hesitate to cont

Thank you for your inquiry. We have a number of antibodies in our catalog that might be of interest to you. I would like to direct you to the search facility on our website (www.abcam.com). Please enter the name of the protein into the search box at the top of the home page. A list of products will be generated which can be further refined using the filters on the left hand side of the page. Using these filters will help you to identify products that have been tested in the applications and species you are interested in. I have conducted a search for you and found one antibody which may be interest to you: ab105156 - Rat monoclonal [CI:A3-1] to F4/80 (Phycoerythrin) - it recognizes mouse F4/80 antigen. I hope this information is helpful to you. If you have specific questions about the products identified during the search, please do not hesitate to contact me.