Key features and details
- PE Sheep polyclonal to FITC
- Suitable for: WB, ELISA, ICC/IF, Dot blot
- Reacts with: Species independent
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG
Product namePE Anti-FITC antibody
See all FITC primary antibodies
DescriptionPE Sheep polyclonal to FITC
ConjugationPE. Ex: 488nm, Em: 575nm
SpecificityAb25539 recognises FITC.
Tested applicationsSuitable for: WB, ELISA, ICC/IF, Dot blotmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule: Fluorescein Isothiocyanate (Isomer I).
Ab25539 shows cross absorption with mouse, human and rat serum proteins.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Also contains a stabilizing agent.
Concentration information loading...
Purification notesAb25539 is purified by affinity chromatography.
Primary antibody notesAb25539 shows cross absorption with mouse, human and rat serum proteins.
Our Abpromise guarantee covers the use of ab25539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
|ELISA||Use at an assay dependent dilution.|
|ICC/IF||Use µg for 10 cells.|
|Dot blot||Use at an assay dependent dilution.|
RelevanceFITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
- Fluorescein Isothiocyanate antibody
ab25539 has been referenced in 1 publication.
- Ma X et al. Fraxin Alleviates LPS-Induced ARDS by Downregulating Inflammatory Responses and Oxidative Damages and Reducing Pulmonary Vascular Permeability. Inflammation 42:1901-1912 (2019). PubMed: 31273573