Recombinant PE Anti-Gelsolin antibody [EPR1942] (ab225096)
![Flow Cytometry - PE Anti-Gelsolin antibody [EPR1942] (ab225096)](https://www.abcam.com/ps/products/225/ab225096/Images/ab225096-312700-anti-gelsolin-antibody-epr1942-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-Gelsolin antibody [EPR1942] (ab225096)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR1942] to Gelsolin
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
PE Anti-Gelsolin antibody [EPR1942]
See all Gelsolin primary antibodies -
Description
PE Rabbit monoclonal [EPR1942] to Gelsolin -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Gelsolin aa 450-550 (internal sequence). The exact sequence is proprietary.
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Positive control
- Flow Cyt: MCF7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1942 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab225096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/1000. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis. -
Tissue specificity
Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells. -
Involvement in disease
Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure. -
Sequence similarities
Belongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats. -
Post-translational
modificationsPhosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids. -
Cellular localization
Cytoplasm > cytoskeleton and Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2934 Human
- Omim: 137350 Human
- SwissProt: P06396 Human
- Unigene: 522373 Human
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Alternative names
- Actin depolymerizing factor antibody
- Actin-depolymerizing factor antibody
- ADF antibody
see all
Images
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Flow Cytometry - PE Anti-Gelsolin antibody [EPR1942] (ab225096)
Overlay histogram showing MCF7 cells stained with ab225096 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225096, 1/1000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
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![PE Anti-Gelsolin antibody [EPR1942] (ab225096)](https://www.abcam.com/ps/products/225/ab225096/Images/ab225096-3-benefits-of-recombinant-antibodies.png)
PE Anti-Gelsolin antibody [EPR1942] (ab225096)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab225096 has not yet been referenced specifically in any publications.
