RecombinantRabMAb

Recombinant PE Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab209449)

Reviews (1) Submit a question
Immunocytochemistry/ Immunofluorescence - PE Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab209449)
  • Western blot - PE Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab209449)
  • Flow Cytometry - PE Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab209449)
  • PE Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab209449)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [EPR3915] to Glucose Transporter GLUT1
  • Suitable for: Flow Cyt, ICC/IF, WB
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Overview

  • Product name

    PE Anti-Glucose Transporter GLUT1 antibody [EPR3915]
    See all Glucose Transporter GLUT1 primary antibodies

  • Description

    PE Rabbit monoclonal [EPR3915] to Glucose Transporter GLUT1

  • Host species

    Rabbit

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Glucose Transporter GLUT1 aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: P11166
    (Peptide available as ab202335)

  • Positive control

    • Flow Cyt: HepG2 cells ICC/IF: HepG2 cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab209449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/2500.

ab209478 - Rabbit monoclonal IgG (Phycoerythrin), is suitable for use as an isotype control with this antibody.
ICC/IF 1/500.

This product gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa.Can be blocked with Glucose Transporter GLUT1 peptide (ab202335).

Target

  • Function

    Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.

  • Tissue specificity

    Expressed at variable levels in many human tissues.

  • Involvement in disease

    Defects in SLC2A1 are the cause of glucose transporter type 1 deficiency syndrome (GLUT1DS) [MIM:606777]; also known as blood-brain barrier glucose transport defect. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
    Defects in SLC2A1 are the cause of dystonia type 18 (DYT18) [MIM:612126]. DYT18 is an exercise-induced paroxysmal dystonia/dyskinesia. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT18 is characterized by attacks of involuntary movements triggered by certain stimuli such as sudden movement or prolonged exercise. In some patients involuntary exertion-induced dystonic, choreoathetotic, and ballistic movements may be associated with macrocytic hemolytic anemia.

  • Sequence similarities

    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Cell membrane. Melanosome. Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
    • CSE antibody
    • DYT17 antibody
    • DYT18 antibody
    • DYT9 antibody
    • EIG12 antibody
    • erythrocyte/brain antibody
    • Erythrocyte/hepatoma glucose transporter antibody
    • facilitated glucose transporter member 1 antibody
    • Glucose transporter 1 antibody
    • Glucose transporter type 1 antibody
    • Glucose transporter type 1, erythrocyte/brain antibody
    • GLUT antibody
    • GLUT-1 antibody
    • GLUT1 antibody
    • GLUT1DS antibody
    • GLUTB antibody
    • GT1 antibody
    • GTG1 antibody
    • Gtg3 antibody
    • GTR1_HUMAN antibody
    • HepG2 glucose transporter antibody
    • HTLVR antibody
    • Human T cell leukemia virus (I and II) receptor antibody
    • MGC141895 antibody
    • MGC141896 antibody
    • PED antibody
    • RATGTG1 antibody
    • Receptor for HTLV 1 and HTLV 2 antibody
    • SLC2A1 antibody
    • Solute carrier family 2 (facilitated glucose transporter), member 1 antibody
    • Solute carrier family 2 antibody
    • Solute carrier family 2, facilitated glucose transporter member 1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - PE Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab209449)
    Immunocytochemistry/ Immunofluorescence - PE Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab209449)

    ab209449 staining Glucose Transporter GLUT1 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209449 at 1/500 dilution (Pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Western blot - PE Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab209449)
    Western blot - PE Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab209449)

    Clone EPR3915 has been knock-out validated in its unconjugated form. Please refer to ab115730 datasheet for experimental details.

  • Flow Cytometry - PE Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab209449)
    Flow Cytometry - PE Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab209449)

    Overlay histogram showing HepG2 cells stained with ab209449 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol (-20°C) for 30 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209449, 1/2500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycorythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20 mW Solid State Blue Laser (488nm) and 585/40 bandpass filter.

  • PE Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab209449)
    PE Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab209449)

References (0)

Publishing research using ab209449? Please let us know so that we can cite the reference in this datasheet.

ab209449 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Flow Cytometry abreview for Anti-Glucose Transporter GLUT1 antibody [EPR3915] (Phycoerythrin)

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Sample
Mouse Cell (Lewis lung carcinoma)
Permeabilization
Yes - Methanol 90%
Gating Strategy
SSC-A and FSC-A equivalent to LL2(LC1)cells
Specification
Lewis lung carcinoma
Fixation
Formaldehyde
Read More

Aline Oliveira

Verified customer

Submitted Aug 02 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com