RecombinantRabMAb

Recombinant PE Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab210583)

Submit a review Submit a question
Flow Cytometry - PE Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab210583)
  • Immunocytochemistry/ Immunofluorescence - PE Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab210583)
  • PE Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab210583)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [EP817Y] to Glycogen synthase 1/GYS1
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Overview

  • Product name

    PE Anti-Glycogen synthase 1/GYS1 antibody [EP817Y]
    See all Glycogen synthase 1/GYS1 primary antibodies

  • Description

    PE Rabbit monoclonal [EP817Y] to Glycogen synthase 1/GYS1

  • Host species

    Rabbit

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    within Human Glycogen synthase 1/GYS1 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: P13807

  • Positive control

    • Flow Cyt: HeLa cells ICC/IF: HeLa cells

  • General notes

     This product was previously labelled as Glycogen synthase 1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab210583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.
ICC/IF 1/50.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

Target

  • Function

    Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.

  • Pathway

    Glycan biosynthesis; glycogen biosynthesis.

  • Involvement in disease

    Defects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work.

  • Sequence similarities

    Belongs to the glycosyltransferase 3 family.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Glycogen [starch] synthase antibody
    • Glycogen synthase 1 (muscle) antibody
    • Glycogen synthase 1 antibody
    • GSY antibody
    • GYS antibody
    • Gys1 antibody
    • GYS1_HUMAN antibody
    • muscle antibody
    see all

Images

  • Flow Cytometry - PE Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab210583)
    Flow Cytometry - PE Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab210583)

    Overlay histogram showing HeLa cells stained with ab210583 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol for 30 min at -20°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210583, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50mW Yellow-Green laser (561nm) and 586/15 bandpass filter.

  • Immunocytochemistry/ Immunofluorescence - PE Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab210583)
    Immunocytochemistry/ Immunofluorescence - PE Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab210583)

    ab210583 staining Glycogen synthase 1/GYS1 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab210583 at 1/100 dilution (Pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

  • PE Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab210583)
    PE Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab210583)

References (0)

Publishing research using ab210583? Please let us know so that we can cite the reference in this datasheet.

ab210583 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab210583.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com