Key features and details
- PE Mouse monoclonal [HIR2] to Glycophorin A + B
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2b
Product namePE Anti-Glycophorin A + B antibody [HIR2]
See all Glycophorin A + B primary antibodies
DescriptionPE Mouse monoclonal [HIR2] to Glycophorin A + B
ConjugationPE. Ex: 488nm, Em: 575nm
SpecificityThe antibody recognizes the N-terminal, homologous portion of glycophorins A (GPA) and B (GPB) which are single-pass membrane sialoglycoproteins and it binds strongly to GPA but weakly to GPB.
Species reactivityReacts with: Human
Synthetic peptide N terminal (Human)
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Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA
Concentration information loading...
PurityProtein A purified
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceGlycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPA gene consists of 7 exons and has 97% sequence homology with GYPB from the 5' UTR to the coding sequence encoding the first 45 amino acids. GYPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are resulted from gene recombinations between GYPA and GYPB. These antigens are expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).
Cellular localizationType I membrane protein.
- CD235a antibody
- CD235b antibody
- Erythroid lineage specific membrane sialoglycoprotein antibody
ab26016 has been referenced in 1 publication.
- Nixon AML et al. A rapid in vitro methodology for simultaneous target discovery and antibody generation against functional cell subpopulations. Sci Rep 9:842 (2019). PubMed: 30696911