PE Anti-Glycophorin A + B antibody [HIR2] (ab26016)
Key features and details
- PE Mouse monoclonal [HIR2] to Glycophorin A + B
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
PE Anti-Glycophorin A + B antibody [HIR2]
See all Glycophorin A + B primary antibodies -
Description
PE Mouse monoclonal [HIR2] to Glycophorin A + B -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
The antibody recognizes the N-terminal, homologous portion of glycophorins A (GPA) and B (GPB) which are single-pass membrane sialoglycoproteins and it binds strongly to GPA but weakly to GPB. -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide N terminal (Human)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HIR2 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Application notes
Flow Cyt: Use 20µl for 106 cells or 100µl whole blood.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPA gene consists of 7 exons and has 97% sequence homology with GYPB from the 5' UTR to the coding sequence encoding the first 45 amino acids. GYPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are resulted from gene recombinations between GYPA and GYPB. These antigens are expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative). -
Cellular localization
Type I membrane protein. -
Database links
- Entrez Gene: 2993 Human
- Entrez Gene: 2994 Human
- SwissProt: P02724 Human
- SwissProt: P06028 Human
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Alternative names
- CD235a antibody
- CD235b antibody
- Erythroid lineage specific membrane sialoglycoprotein antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab26016 has been referenced in 1 publication.
- Nixon AML et al. A rapid in vitro methodology for simultaneous target discovery and antibody generation against functional cell subpopulations. Sci Rep 9:842 (2019). PubMed: 30696911