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  1. Link

    pe-glycophorin-a--b-antibody-hir2-ab26016.pdf

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Cardiovascular Blood Blood Cell Antigens RBC Antigens
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PE Anti-Glycophorin A + B antibody [HIR2] (ab26016)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (1)

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Key features and details

  • PE Mouse monoclonal [HIR2] to Glycophorin A + B
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Isotype: IgG2b

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Conjugation
Product image
PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)

View more associated products

Overview

  • Product name

    PE Anti-Glycophorin A + B antibody [HIR2]
    See all Glycophorin A + B primary antibodies
  • Description

    PE Mouse monoclonal [HIR2] to Glycophorin A + B
  • Host species

    Mouse
  • Conjugation

    PE. Ex: 488nm, Em: 575nm
  • Specificity

    The antibody recognizes the N-terminal, homologous portion of glycophorins A (GPA) and B (GPB) which are single-pass membrane sialoglycoproteins and it binds strongly to GPA but weakly to GPB.
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide N terminal (Human)

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    HIR2
  • Isotype

    IgG2b
  • Research areas

    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Blood Group Antigens

Associated products

  • Alternative Versions

    • Anti-Glycophorin A + B antibody [HIR2] (ab15009)
    • FITC Anti-Glycophorin A + B antibody [HIR2] (ab52452)
    • Biotin Anti-Glycophorin A + B antibody [HIR2] (ab93548)
  • Isotype control

    • PE Mouse IgG2b [PLPV219] - Isotype Control (ab91532)

Applications

  • Application notes
    Flow Cyt: Use 20µl for 106 cells or 100µl whole blood.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPA gene consists of 7 exons and has 97% sequence homology with GYPB from the 5' UTR to the coding sequence encoding the first 45 amino acids. GYPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are resulted from gene recombinations between GYPA and GYPB. These antigens are expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).
    • Cellular localization

      Type I membrane protein.
    • Database links

      • Entrez Gene: 2993 Human
      • Entrez Gene: 2994 Human
      • SwissProt: P02724 Human
      • SwissProt: P06028 Human
      • Unigene: 434973 Human
      • Unigene: 654368 Human
      • Alternative names

        • CD235a antibody
        • CD235b antibody
        • Erythroid lineage specific membrane sialoglycoprotein antibody
        • Glycophorin Erik antibody
        • Glycophorin HeP2 antibody
        • Glycophorin MiI antibody
        • Glycophorin MiIII antibody
        • Glycophorin MiV antibody
        • Glycophorin MiVI antibody
        • Glycophorin MiX antibody
        • Glycophorin SAT antibody
        • Glycophorin Sta type C antibody
        • GPA antibody
        • GPB antibody
        • GPB.NY antibody
        • GPErik antibody
        • GpMiIII antibody
        • GPSAT antibody
        • GYPA antibody
        • GYPB antibody
        • GYPHe.NY antibody
        • HGpMiIII antibody
        • HGpMiV antibody
        • HGpMiVI antibody
        • HGpMiX antibody
        • HGpMiXI antibody
        • HGpSta(C) antibody
        • Mi.V glycoprotein (24 AA) antibody
        • MN antibody
        • MN sialoglycoprotein antibody
        • MNS antibody
        • PAS 2 antibody
        • PAS 3 antibody
        • PAS2 antibody
        • PAS3 antibody
        • Sialoglycoprotein alpha antibody
        • Sialoglycoprotein delta antibody
        • SS active sialoglycoprotein antibody
        • SS antibody
        see all

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab26016? Please let us know so that we can cite the reference in this datasheet.

      ab26016 has been referenced in 1 publication.

      • Nixon AML  et al. A rapid in vitro methodology for simultaneous target discovery and antibody generation against functional cell subpopulations. Sci Rep 9:842 (2019). PubMed: 30696911

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      I would like to enquire a question. Are there any antibody of Anti-Glycophorin A antibody [HIR2] (ab15009) conjugated with FITC and antibody of Anti-Glycophorin A antibody [HIR2] (ab15009) conjugated with Phycoerythrin?
      If the antibody I mentioned is not available, is it possible to obtain those antibodies I mentioned, meaning specifically made it?
      If there is the antibody I mentioned or it is possible to specifically made the antibodies I mentioned, I would like to enquire a few questions as listed below:-
      (a) What is the concentration of the antibody mentioned?
      (b) What would the price be?
      (c) When will it be able to arrive if I were to order it by this week (Address: London, United Kingdom)?
      In addition, I would like to know the concentration for these antibodies as well.
      (a) Anti-Glycophorin A antibody [HIR2] (FITC) - Aminoterminal end (ab52452)
      (b) Anti-Glycophorin A + B antibody [HIR2] (Phycoerythrin) (ab26016)
      Thank you for taking up your time.
      Hope to hear from you soon.

      Read More

      Abcam community

      Verified customer

      Asked on Jun 12 2012

      Answer

      Thank you for contacting us.

      We have 4, FITC conjugated anti Glycophorin A antibodies.

      https://www.abcam.com/Glycophorin-A-antibody-YTH89-1-FITC-ab28082.html
      https://www.abcam.com/Glycophorin-A-antibody-FITC-ab112201.html
      https://www.abcam.com/Glycophorin-A-antibody-HIR2-FITC-Aminoterminal-end-ab52452.html
      https://www.abcam.com/Glycophorin-A-antibody-CLB-AME-1-FITC-ab51531.html

      3 Phycoerythrin conjugated abs

      https://www.abcam.com/Glycophorin-A-antibody-CLB-AME-1-Phycoerythrin-ab51632.html
      https://www.abcam.com/Glycophorin-A-antibody-YTH89-1-Phycoerythrin-ab33387.html
      https://www.abcam.com/Glycophorin-A-B-antibody-HIR2-Phycoerythrin-ab26016.html

      The price for these antibodies for 1 vial would be

      ab28082; £283 per 100 microgram
      ab51632: £227 per 500 micro litre
      ab112201; £227 per 100 microgram
      ab33387: £227 50 tests
      ab52452: £227 per 100 tests
      ab51531; £227 per 100 tests
      ab26016: £227 per 100 tests

      ab26016 the concentration is 0.01mg/ml

      All other information is present on the datasheets. The product will be dispatched same day for next day delivery.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on Jun 12 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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