RecombinantRabMAb

Recombinant PE Anti-GSK3 beta antibody [Y174] (ab210619)

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Immunocytochemistry/ Immunofluorescence - PE Anti-GSK3 beta antibody [Y174] (ab210619)
  • Flow Cytometry - PE Anti-GSK3 beta antibody [Y174] (ab210619)
  • PE Anti-GSK3 beta antibody [Y174] (ab210619)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [Y174] to GSK3 beta
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Overview

  • Product name

    PE Anti-GSK3 beta antibody [Y174]
    See all GSK3 beta primary antibodies

  • Description

    PE Rabbit monoclonal [Y174] to GSK3 beta

  • Host species

    Rabbit

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Specificity

    This antibody may also detect the splice isoform 2 based on sequence homology.

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide within Human GSK3 beta aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P49841

  • Positive control

    • Flow Cytometry: MCF7 cells ICC/IF: MCF7 cells

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Applications

Our Abpromise guarantee covers the use of ab210619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.

This product gave a positive signal in MCF7 cells fixed with 100% methanol (5 min)
Flow Cyt 1/5000.

Target

  • Function

    Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.

  • Tissue specificity

    Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.

  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216.

  • Cellular localization

    Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Glycogen Synthase Kinase 3 Beta antibody
      • Glycogen synthase kinase-3 beta antibody
      • GSK 3 beta antibody
      • GSK-3 beta antibody
      • GSK3B antibody
      • GSK3B_HUMAN antibody
      • GSK3beta isoform antibody
      • Serine/threonine-protein kinase GSK3B antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - PE Anti-GSK3 beta antibody [Y174] (ab210619)
      Immunocytochemistry/ Immunofluorescence - PE Anti-GSK3 beta antibody [Y174] (ab210619)

      ab210619 staining GSK3 beta in MCF7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab210619 at 1/1000 dilution (pseudocolored in green). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Flow Cytometry - PE Anti-GSK3 beta antibody [Y174] (ab210619)
      Flow Cytometry - PE Anti-GSK3 beta antibody [Y174] (ab210619)

      Overlay histogram showing MCF7 cells stained with ab210619 (red line). The cells were fixed with 4% formaldehyde and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210619, 1/5000 dilution) for 30 min at 22°C.          

      Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. 

      Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter.               

    • PE Anti-GSK3 beta antibody [Y174] (ab210619)
      PE Anti-GSK3 beta antibody [Y174] (ab210619)

    References (0)

    Publishing research using ab210619? Please let us know so that we can cite the reference in this datasheet.

    ab210619 has not yet been referenced specifically in any publications.

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