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  1. Link

    pe-ha-tag-antibody-16b12-ab72479.pdf

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PE Anti-HA tag antibody [16B12] (ab72479)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (2)

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Abpromise

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Find out more.

Flow Cytometry - PE Anti-HA tag antibody [16B12] (ab72479)

    Key features and details

    • PE Mouse monoclonal [16B12] to HA tag
    • Suitable for: Flow Cyt
    • Reacts with: Species independent
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG1

    Overview

    • Product name

      PE Anti-HA tag antibody [16B12]
      See all HA tag primary antibodies
    • Description

      PE Mouse monoclonal [16B12] to HA tag
    • Host species

      Mouse
    • Conjugation

      PE. Ex: 488nm, Em: 575nm
    • Specificity

      ab72479 recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Species independent
    • Immunogen

      Raised against the twelve amino acid peptide CYPYDVPDYASL.

    • Epitope

      ab72479 recognizes the influenza hemagglutinin epitope (YPYDVPDYA).
    • General notes

      Excitation max. lambda: 565>498 nm Emission max. lambda: 578 nm.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      Preservative: 0.013% Sodium azide
      Constituents: 0.1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate
    • Concentration information loading...
    • Purity

      Size exclusion
    • Purification notes

      Size exclusion chromatography as well as proprietary methods for purifying the final materials were used.
    • Clonality

      Monoclonal
    • Clone number

      16B12
    • Isotype

      IgG1
    • Research areas

      • Tags & Cell Markers
      • Epitope Tags
      • HA Tag

    Associated products

    • Alternative Versions

      • PerCP Anti-HA tag antibody [16B12] (ab117491)
      • DyLight® 550 Anti-HA tag antibody [16B12] (ab117502)
      • DyLight® 650 Anti-HA tag antibody [16B12] (ab117515)
      • Alkaline Phosphatase Anti-HA tag antibody [16B12] (ab81656)
    • Isotype control

      • PE Mouse IgG1 [B11/6] - Isotype Control (ab91357)

    Applications

    Our Abpromise guarantee covers the use of ab72479 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use at an assay dependent concentration.

    ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Relevance

      Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
    • Alternative names

      • HA epitope tag antibody
      • HA1 antibody
      • HA2 antibody
      • hemagglutinin antibody
      • Hemagglutinin HA1 chain antibody
      • Hemagglutinin HA2 chain antibody
      see all

    Images

    • Flow Cytometry - PE Anti-HA tag antibody [16B12] (ab72479)
      Flow Cytometry - PE Anti-HA tag antibody [16B12] (ab72479)

      Flow cytometry analysis of a recombinant (N-terminus HA-tag) transmembrane cell surface receptor in CHO cells using ab72479 (blue). Black - Mouse IgG1 Phycoerythrin isotype control.

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (2)

    Publishing research using ab72479? Please let us know so that we can cite the reference in this datasheet.

    ab72479 has been referenced in 2 publications.

    • Takano T  et al. Dectin-1 intracellular domain determines species-specific ligand spectrum by modulating receptor sensitivity. J Biol Chem 292:16933-16941 (2017). PubMed: 28848046
    • Yugawa T  et al. Noncanonical NOTCH signaling limits self-renewal of human epithelial and induced pluripotent stem cells through ROCK activation. Mol Cell Biol 33:4434-47 (2013). PubMed: 24019071

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    What is the typical conjugate:ab ratio for these products?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 18 2013

    Answer

    ab72479:
    Like most phycobiliprotein conjugates, the ratio of the fluor to the antibody (F/P) can vary slightly from lot to lot due to the nature of the conjugation chemistry and the methods used to characterize the final product, but it is typically around 1:1. The F/P for the latest batch of the D5-1722 mouse anti-HA-RPE is 0.83 PE/Ab, which essentially falls into that 1:1 zone

    Read More

    Abcam Scientific Support

    Answered on Sep 19 2013

    Question

    I was just wondering if the PE-conjugated anti-HA antibody 16B12 can be used for intracellular staining, for use in flow cytometry.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 04 2013

    Answer

    ab72479 recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus. You should be able to use it for intracellular staining or extracellular staining

    Read More

    Abcam Scientific Support

    Answered on Mar 04 2013

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