PE Anti-HA tag antibody [16B12] (ab72479)

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Flow Cytometry - PE Anti-HA tag antibody [16B12] (ab72479)

    Key features and details

    • PE Mouse monoclonal [16B12] to HA tag
    • Suitable for: Flow Cyt
    • Reacts with: Species independent
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG1

    Overview

    • Product name

      PE Anti-HA tag antibody [16B12]
      See all HA tag primary antibodies

    • Description

      PE Mouse monoclonal [16B12] to HA tag

    • Host species

      Mouse

    • Conjugation

      PE. Ex: 488nm, Em: 575nm

    • Specificity

      ab72479 recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Species independent

    • Immunogen

      Raised against the twelve amino acid peptide CYPYDVPDYASL.

    • Epitope

      ab72479 recognizes the influenza hemagglutinin epitope (YPYDVPDYA).

    • General notes

      Excitation max. lambda: 565>498 nm Emission max. lambda: 578 nm.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      Preservative: 0.013% Sodium azide
      Constituents: 0.1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate
    • Concentration information loading...

    • Purity

      Size exclusion

    • Purification notes

      Size exclusion chromatography as well as proprietary methods for purifying the final materials were used.

    • Clonality

      Monoclonal

    • Clone number

      16B12

    • Isotype

      IgG1

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab72479 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use at an assay dependent concentration.

    ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     
    Notes
    Flow Cyt
    Use at an assay dependent concentration.

    ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Relevance

      Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    • Alternative names

      • HA epitope tag antibody
      • HA1 antibody
      • HA2 antibody
      • hemagglutinin antibody
      • Hemagglutinin HA1 chain antibody
      • Hemagglutinin HA2 chain antibody
      see all

    Images

    • Flow Cytometry - PE Anti-HA tag antibody [16B12] (ab72479)
      Flow Cytometry - PE Anti-HA tag antibody [16B12] (ab72479)

      Flow cytometry analysis of a recombinant (N-terminus HA-tag) transmembrane cell surface receptor in CHO cells using ab72479 (blue). Black - Mouse IgG1 Phycoerythrin isotype control.

    References (2)

    Publishing research using ab72479? Please let us know so that we can cite the reference in this datasheet.

    ab72479 has been referenced in 2 publications.

    • Takano T  et al. Dectin-1 intracellular domain determines species-specific ligand spectrum by modulating receptor sensitivity. J Biol Chem 292:16933-16941 (2017). PubMed: 28848046
    • Yugawa T  et al. Noncanonical NOTCH signaling limits self-renewal of human epithelial and induced pluripotent stem cells through ROCK activation. Mol Cell Biol 33:4434-47 (2013). PubMed: 24019071

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    What is the typical conjugate:ab ratio for these products?

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    Answer

    ab72479:
    Like most phycobiliprotein conjugates, the ratio of the fluor to the antibody (F/P) can vary slightly from lot to lot due to the nature of the conjugation chemistry and the methods used to characterize the final product, but it is typically around 1:1. The F/P for the latest batch of the D5-1722 mouse anti-HA-RPE is 0.83 PE/Ab, which essentially falls into that 1:1 zone

    Read More

    Question

    I was just wondering if the PE-conjugated anti-HA antibody 16B12 can be used for intracellular staining, for use in flow cytometry.

    Read More
    Answer

    ab72479 recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus. You should be able to use it for intracellular staining or extracellular staining

    Read More

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