PE Anti-HA tag antibody [16B12] (ab72479)
![Flow Cytometry - PE Anti-HA tag antibody [16B12] (ab72479)](https://www.abcam.com/ps/products/72/ab72479/Images/ab72479-241227-anti-ha-tag-antibody-16b12-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-HA tag antibody [16B12] (ab72479)")
Key features and details
- PE Mouse monoclonal [16B12] to HA tag
- Suitable for: Flow Cyt
- Reacts with: Species independent
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-HA tag antibody [16B12]
See all HA tag primary antibodies -
Description
PE Mouse monoclonal [16B12] to HA tag -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
ab72479 recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Raised against the twelve amino acid peptide CYPYDVPDYASL.
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Epitope
ab72479 recognizes the influenza hemagglutinin epitope (YPYDVPDYA). -
General notes
Excitation max. lambda: 565>498 nm Emission max. lambda: 578 nm.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.013% Sodium azide
Constituents: 0.1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
Size exclusion chromatography as well as proprietary methods for purifying the final materials were used. -
Clonality
Monoclonal -
Clone number
16B12 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab72479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
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Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
Alternative names
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
Images
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Flow Cytometry - PE Anti-HA tag antibody [16B12] (ab72479)
Flow cytometry analysis of a recombinant (N-terminus HA-tag) transmembrane cell surface receptor in CHO cells using ab72479 (blue). Black - Mouse IgG1 Phycoerythrin isotype control.
References (2)
ab72479 has been referenced in 2 publications.
- Takano T et al. Dectin-1 intracellular domain determines species-specific ligand spectrum by modulating receptor sensitivity. J Biol Chem 292:16933-16941 (2017). PubMed: 28848046
- Yugawa T et al. Noncanonical NOTCH signaling limits self-renewal of human epithelial and induced pluripotent stem cells through ROCK activation. Mol Cell Biol 33:4434-47 (2013). PubMed: 24019071
