Recombinant PE Anti-Hes1 antibody [EPR4226] (ab225087)
![Flow Cytometry (Intracellular) - PE Anti-Hes1 antibody [EPR4226] (ab225087)](https://www.abcam.com/ps/products/225/ab225087/Images/ab225087-341210-ab225087FLOW1.jpg "Flow Cytometry (Intracellular) - PE Anti-Hes1 antibody [EPR4226] (ab225087)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR4226] to Hes1
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
Overview
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Product name
PE Anti-Hes1 antibody [EPR4226]
See all Hes1 primary antibodies -
Description
PE Rabbit monoclonal [EPR4226] to Hes1 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (intra): SHSY5Y cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EPR4226 -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab225087 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/1000.
The cellular localisation of this product has been verified in ICC/IF. |
| Notes |
|---|
|
Flow Cyt (Intra)
1/1000. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain. -
Domain
Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3280 Human
- Entrez Gene: 15205 Mouse
- Entrez Gene: 29577 Rat
- Omim: 139605 Human
- SwissProt: Q14469 Human
- SwissProt: P35428 Mouse
- SwissProt: Q04666 Rat
- Unigene: 250666 Human
see all -
Alternative names
- bHLHb39 antibody
- C-HAIRY1 antibody
- c-hairy1A antibody
see all
Images
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Flow Cytometry (Intracellular) - PE Anti-Hes1 antibody [EPR4226] (ab225087)
Overlay histogram showing SHSY5Y cells stained with ab225087 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225087, 1/1000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
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![PE Anti-Hes1 antibody [EPR4226] (ab225087)](https://www.abcam.com/ps/products/225/ab225087/Images/ab225087-3-benefits-of-recombinant-antibodies.png)
PE Anti-Hes1 antibody [EPR4226] (ab225087)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab225087 has not yet been referenced specifically in any publications.
