Recombinant PE Anti-Hes1 antibody [EPR4226] (ab225087)
![Flow Cytometry - PE Anti-Hes1 antibody [EPR4226] (ab225087)](https://www.abcam.com/ps/products/225/ab225087/Images/ab225087-341210-ab225087FLOW1.jpg "Flow Cytometry - PE Anti-Hes1 antibody [EPR4226] (ab225087)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR4226] to Hes1
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
-
Product name
PE Anti-Hes1 antibody [EPR4226]
See all Hes1 primary antibodies -
Description
PE Rabbit monoclonal [EPR4226] to Hes1 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Synthetic peptide within Human Hes1 aa 200-300. The exact sequence is proprietary.
-
Positive control
- Flow Cyt: SHSY5Y cells
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EPR4226 -
Research areas
Associated products
-
Alternative Versions
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab225087 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/1000. The cellular localisation of this product has been verified in ICC/IF. |
Target
-
Function
Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain. -
Domain
Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 3280 Human
- Entrez Gene: 15205 Mouse
- Entrez Gene: 29577 Rat
- Omim: 139605 Human
- SwissProt: Q14469 Human
- SwissProt: P35428 Mouse
- SwissProt: Q04666 Rat
- Unigene: 250666 Human
see all -
Alternative names
- bHLHb39 antibody
- C-HAIRY1 antibody
- c-hairy1A antibody
see all
Images
-
Flow Cytometry - PE Anti-Hes1 antibody [EPR4226] (ab225087)
Overlay histogram showing SHSY5Y cells stained with ab225087 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225087, 1/1000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
-
![PE Anti-Hes1 antibody [EPR4226] (ab225087)](https://www.abcam.com/ps/products/225/ab225087/Images/ab225087-3-benefits-of-recombinant-antibodies.png)
PE Anti-Hes1 antibody [EPR4226] (ab225087)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab225087 has not yet been referenced specifically in any publications.
