PE Anti-HLA Class I antibody [MEM-123] (ab58998)

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Flow Cytometry - PE Anti-HLA Class I antibody [MEM-123] (ab58998)

    Key features and details

    • PE Mouse monoclonal [MEM-123] to HLA Class I
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG3

    Overview

    • Product name

      PE Anti-HLA Class I antibody [MEM-123]
      See all HLA Class I primary antibodies

    • Description

      PE Mouse monoclonal [MEM-123] to HLA Class I

    • Host species

      Mouse

    • Conjugation

      PE. Ex: 488nm, Em: 575nm

    • Specificity

      ab58998 reacts with all human classical MHC Class I molecules in native cell-surface forms as well as with human HLA G cDNA transfected cells. ab58998 completely blocks binding of classical W6/32 to surface expressed HLA G.

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      COS7 African green monkey kidney cells

    • General notes

      Do not use after expiration date stamped on vial label. Short term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

      The purified antibody was conjugated with R Phycoerythrin (PE) under optimum conditions. The conjugate was purified by size exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 7.4
      Preservative: 0.097% Sodium azide
      Constituent: 0.2% BSA

      0.2% high grade protease free BSA
    • Concentration information loading...

    • Purity

      Size exclusion

    • Purification notes

      The antibody was >95% pure as determined from SDS-PAGE (purified using protein A affinity chromatography prior to conjugation).

    • Clonality

      Monoclonal

    • Clone number

      MEM-123

    • Isotype

      IgG3

    • Research areas

    Associated products

    Applications

    Our Abpromise guarantee covers the use of ab58998 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use at an assay dependent concentration.

    Target

    Images

    • Flow Cytometry - PE Anti-HLA Class I antibody [MEM-123] (ab58998)
      Flow Cytometry - PE Anti-HLA Class I antibody [MEM-123] (ab58998)
      Flow Cytometry analysis of K562 cells labeling HLA Class I with Anti-HLA Class I antibody [MEM-123] (Phycoerythrin) (ab58998).

    References (1)

    Publishing research using ab58998? Please let us know so that we can cite the reference in this datasheet.

    ab58998 has been referenced in 1 publication.

    • Toyoshima K  et al. Analysis of circulating tumor cells derived from advanced gastric cancer. Int J Cancer 137:991-8 (2015). Flow Cyt ; Human . PubMed: 25622566

    Customer reviews and Q&As

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    Question

    Would like to use this antibody in IHC-Fr to stain human cells injected into mouse heart tissue.

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    Answer


    DISCOUNT CODE: ***
    Expiration date: June 22, 2012

    This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for ab58998 in IHC-Frand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com