PE Anti-HLA Class I antibody [MEM-123] (ab58998)
Key features and details
- PE Mouse monoclonal [MEM-123] to HLA Class I
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG3
Related conjugates and formulations
Overview
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Product name
PE Anti-HLA Class I antibody [MEM-123]
See all HLA Class I primary antibodies -
Description
PE Mouse monoclonal [MEM-123] to HLA Class I -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
ab58998 reacts with all human classical MHC Class I molecules in native cell-surface forms as well as with human HLA G cDNA transfected cells. ab58998 completely blocks binding of classical W6/32 to surface expressed HLA G. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to African green monkey HLA Class I. COS-7 cells
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Positive control
- Flow Cyt: K562 cells
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General notes
Do not use after expiration date stamped on vial label. Short term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.The purified antibody was conjugated with R Phycoerythrin (PE) under optimum conditions. The conjugate was purified by size exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituent: 0.2% BSA
0.2% high grade protease free BSA -
Concentration information loading...
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Purity
Size exclusion -
Purification notes
The antibody was >95% pure as determined from SDS-PAGE (purified using protein A affinity chromatography prior to conjugation). -
Clonality
Monoclonal -
Clone number
MEM-123 -
Isotype
IgG3 -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab58998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use a concentration of 2 - 4 µg/ml.
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Notes |
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Flow Cyt
Use a concentration of 2 - 4 µg/ml. |
Target
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Relevance
HLA CLass I is involved in the presentation of foreign antigens to the immune system. -
Cellular localization
Plasma membrane -
Database links
- Entrez Gene: 3105 Human
- Entrez Gene: 3133 Human
- Entrez Gene: 3134 Human
- Entrez Gene: 3135 Human
- Omim: 142800 Human
- SwissProt: O15506 Human
- SwissProt: P04439 Human
- SwissProt: P13747 Human
see all -
Alternative names
- DKFZp686P19218 antibody
- EA1.2 antibody
- EA2.1 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab58998 has been referenced in 1 publication.
- Toyoshima K et al. Analysis of circulating tumor cells derived from advanced gastric cancer. Int J Cancer 137:991-8 (2015). Flow Cyt ; Human . PubMed: 25622566