PE Anti-HLA Class I antibody [MEM-147] (ab52451)
Key features and details
- PE Mouse monoclonal [MEM-147] to HLA Class I
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-HLA Class I antibody [MEM-147]
See all HLA Class I primary antibodies -
Description
PE Mouse monoclonal [MEM-147] to HLA Class I -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
ab5451 recognises all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types. ab52451 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. -
Species reactivity
Reacts with: Human -
Immunogen
PHA-activated peripheral blood lymphocytes.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituent: 0.2% BSA
BSA (high-grade protease free), -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab52451 was purified by Protein G prior to conjugation. After conjugation, it was purified by site exclusion chromatography. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
MEM-147 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
Target
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Relevance
HLA CLass I is involved in the presentation of foreign antigens to the immune system. -
Cellular localization
Plasma membrane -
Database links
- Entrez Gene: 3105 Human
- Entrez Gene: 3133 Human
- Entrez Gene: 3134 Human
- Entrez Gene: 3135 Human
- Omim: 142800 Human
- SwissProt: O15506 Human
- SwissProt: P01891 Human
- SwissProt: P01892 Human
see all -
Alternative names
- DKFZp686P19218 antibody
- EA1.2 antibody
- EA2.1 antibody
see all
References (0)
ab52451 has not yet been referenced specifically in any publications.