PE Anti-HLA Class I antibody [MEM-147] (ab52451)

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Key features and details

  • PE Mouse monoclonal [MEM-147] to HLA Class I
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Isotype: IgG1

Overview

  • Product name

    PE Anti-HLA Class I antibody [MEM-147]
    See all HLA Class I primary antibodies

  • Description

    PE Mouse monoclonal [MEM-147] to HLA Class I

  • Host species

    Mouse

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Specificity

    ab5451 recognises all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types. ab52451 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A.

  • Species reactivity

    Reacts with: Human

  • Immunogen

    PHA-activated peripheral blood lymphocytes.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituent: 0.2% BSA

    BSA (high-grade protease free),
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    ab52451 was purified by Protein G prior to conjugation. After conjugation, it was purified by site exclusion chromatography. Purity >95% by SDS-PAGE.

  • Clonality

    Monoclonal

  • Clone number

    MEM-147

  • Isotype

    IgG1

  • Research areas

Target

References (0)

Publishing research using ab52451? Please let us know so that we can cite the reference in this datasheet.

ab52451 has not yet been referenced specifically in any publications.

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