PE Anti-HLA Class I antibody [MEM-147] (ab52451)

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Key features and details

  • PE Mouse monoclonal [MEM-147] to HLA Class I
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    PE Anti-HLA Class I antibody [MEM-147]
    See all HLA Class I primary antibodies

  • Description

    PE Mouse monoclonal [MEM-147] to HLA Class I

  • Host species

    Mouse

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Specificity

    ab5451 recognises all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types. ab52451 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A.

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus corresponding to Human HLA Class I. PHA-activated peripheral blood lymphocytes

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituent: 0.2% BSA

    BSA (high-grade protease free),
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    ab52451 was purified by Protein G prior to conjugation. After conjugation, it was purified by site exclusion chromatography. Purity >95% by SDS-PAGE.

  • Clonality

    Monoclonal

  • Clone number

    MEM-147

  • Isotype

    IgG1

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.

ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Notes
Flow Cyt
Use at an assay dependent concentration.

ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

References (0)

Publishing research using ab52451? Please let us know so that we can cite the reference in this datasheet.

ab52451 has not yet been referenced specifically in any publications.

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