PE Anti-HLA G antibody [MEM-G/9] (ab24384)
![Flow Cytometry - PE Anti-HLA G antibody [MEM-G/9] (ab24384)](https://www.abcam.com/ps/products/24/ab24384/Images/ab24384-279897-anti-hla-g-antibody-mem-g-9-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-HLA G antibody [MEM-G/9] (ab24384)")
Key features and details
- PE Mouse monoclonal [MEM-G/9] to HLA G
- Suitable for: Flow Cyt
- Reacts with: Recombinant fragment
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
PE Anti-HLA G antibody [MEM-G/9]
See all HLA G primary antibodies -
Description
PE Mouse monoclonal [MEM-G/9] to HLA G -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
The antibody reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant full length protein (Human) refolded with beta2-microglobulin and peptide.
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General notes
The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MEM-G/9 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use a concentration of 1 - 4 µg/ml.
Do not fix cells. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt
Use a concentration of 1 - 4 µg/ml. Do not fix cells. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells. -
Tissue specificity
Expressed in trophoblasts. -
Sequence similarities
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- B2 microglobulin antibody
- DADB-15K14.8 antibody
- HLA 6.0 antibody
see all
Images
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Flow Cytometry - PE Anti-HLA G antibody [MEM-G/9] (ab24384)Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) PE.
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab24384 has been referenced in 9 publications.
- Nikoo S et al. Culture density of menstrual blood-derived stromal/stem cells determines the quality of T cell responses: An experimental study. Int J Reprod Biomed 19:75-86 (2021). PubMed: 33554005
- Carré T et al. Selection of tumor-resistant variants following sustained natural killer cell-mediated immune stress. Oncol Rep 45:582-594 (2021). PubMed: 33416152
- Dong C et al. Derivation of trophoblast stem cells from naïve human pluripotent stem cells. Elife 9:N/A (2020). PubMed: 32048992
- Salvany-Celades M et al. Three Types of Functional Regulatory T Cells Control T Cell Responses at the Human Maternal-Fetal Interface. Cell Rep 27:2537-2547.e5 (2019). PubMed: 31141680
- Tilburgs T et al. NLRP2 is a suppressor of NF-?B signaling and HLA-C expression in human trophoblasts†,‡. Biol Reprod 96:831-842 (2017). PubMed: 28340094
- Ferreira LM et al. A distant trophoblast-specific enhancer controls HLA-G expression at the maternal-fetal interface. Proc Natl Acad Sci U S A 113:5364-9 (2016). PubMed: 27078102
- Horie A et al. Laeverin/aminopeptidase Q induces trophoblast invasion during human early placentation. Hum Reprod 27:1267-76 (2012). PubMed: 22402206
- Zhao L et al. Reassessment of HLA-G isoform specificity of MEM-G/9 and 4H84 monoclonal antibodies. Tissue Antigens 80:231-8 (2012). PubMed: 22738368
- Menier C et al. Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol 64:315-26 (2003). PubMed: 12590976