Recombinant PE Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab210585)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR5777] to HP1 alpha - Heterochromatin marker
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
Overview
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Product name
PE Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker
See all HP1 alpha primary antibodies -
Description
PE Rabbit monoclonal [EPR5777] to HP1 alpha - Heterochromatin marker -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (intra): HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5777 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
- Alexa Fluor® 488 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab185018)
- HRP Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab197721)
- Alexa Fluor® 647 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab198391)
- Alexa Fluor® 568 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab202533)
- Alexa Fluor® 594 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab203431)
- Alexa Fluor® 555 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab203432)
- Anti-HP1 alpha antibody [EPR5777] - BSA and Azide free (ab226049)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab210585 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/500.
The cellular localisation of this product has been verified in ICC/IF |
Notes |
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Flow Cyt (Intra)
1/500. The cellular localisation of this product has been verified in ICC/IF |
Target
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Function
Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. -
Sequence similarities
Contains 2 chromo domains. -
Post-translational
modificationsPhosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Ubiquitinated. -
Cellular localization
Nucleus. Chromosome. Chromosome > centromere. Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase. - Information by UniProt
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Database links
- Entrez Gene: 23468 Human
- Entrez Gene: 12419 Mouse
- Entrez Gene: 300266 Rat
- Omim: 604478 Human
- SwissProt: P45973 Human
- SwissProt: Q61686 Mouse
- Unigene: 349283 Human
- Unigene: 262059 Mouse
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Alternative names
- Antigen p25 antibody
- CBX5 antibody
- CBX5_HUMAN antibody
see all
Images
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Overlay histogram showing HeLa cells stained with ab210585 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210585, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab210585 has not yet been referenced specifically in any publications.