PE Anti-Human Nuclear Antigen antibody [235-1] (ab215755)
![Flow Cytometry - PE Anti-Human Nuclear Antigen antibody [235-1] (ab215755)](https://www.abcam.com/ps/products/215/ab215755/Images/ab215755-268539-anti-human-nuclear-antigen-antibody-235-1-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-Human Nuclear Antigen antibody [235-1] (ab215755)")
Key features and details
- PE Mouse monoclonal [235-1] to Human Nuclear Antigen
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-Human Nuclear Antigen antibody [235-1]
See all Human Nuclear Antigen primary antibodies -
Description
PE Mouse monoclonal [235-1] to Human Nuclear Antigen -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues and biochemical preparations. This antibody recognizes an antigen associated with the nuclei in human cells. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Non human primates
Does not react with:
Mouse, Rat, Chicken -
Immunogen
Tissue, cells or virus corresponding to Human Nuclear Antigen. Nuclei of human myeloid leukemia biopsy cells
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Positive control
- FACS: MCF7 and HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
235-1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab215755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. 5 µL per test per one million cells in 0.1 mL or 5 µL per 100 µL of whole blood. |
Target
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Alternative names
- HNA antibody
- HuNu antibody
Images
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Flow Cytometry - PE Anti-Human Nuclear Antigen antibody [235-1] (ab215755)
Flow cytometry analysis of MCF7 cells labeling Human Nuclear Antigen using ab215755 at 5 μL per 106 cells (red).
Controls: Cells alone (black) and isotype control (green).
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![Flow Cytometry - PE Anti-Human Nuclear Antigen antibody [235-1] (ab215755)](https://www.abcam.com/ps/products/215/ab215755/Images/ab215755-268538-anti-human-nuclear-antigen-antibody-235-1-phycoerythrin-flow-cytometry.jpg)
Flow Cytometry - PE Anti-Human Nuclear Antigen antibody [235-1] (ab215755)Flow cytometry analysis of HeLa cells labeling Human Nuclear Antigen using ab215755 at 5 μL per 106 cells (red).
Controls: Cells alone (black) and isotype control (green).
Datasheets and documents
References (0)
ab215755 has not yet been referenced specifically in any publications.
