PE Anti-ICAM1 antibody [YN1/1.7.4] (ab25553)
![Flow Cytometry - PE Anti-ICAM1 antibody [YN1/1.7.4] (ab25553)](https://www.abcam.com/ps/products/25/ab25553/Images/ab25553-272406-anti-icam1-antibody-yn1-174-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-ICAM1 antibody [YN1/1.7.4] (ab25553)")
Key features and details
- PE Rat monoclonal [YN1/1.7.4] to ICAM1
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
PE Anti-ICAM1 antibody [YN1/1.7.4]
See all ICAM1 primary antibodies -
Description
PE Rat monoclonal [YN1/1.7.4] to ICAM1 -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to ICAM1. Mouse NS-1 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Also contains a stabilizing agent. -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
YN1/1.7.4 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | (1) |
Use 0.2µg for 106 cells.
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| Notes |
|---|
|
Flow Cyt
Use 0.2µg for 106 cells. |
Target
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Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. -
Sequence similarities
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsMonoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 15894 Mouse
- SwissProt: P13597 Mouse
- Unigene: 435508 Mouse
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Alternative names
- Antigen identified by monoclonal antibody BB2 antibody
- BB 2 antibody
- BB2 antibody
see all
Images
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Flow Cytometry - PE Anti-ICAM1 antibody [YN1/1.7.4] (ab25553)
Flow cytometric analysis of C57BL/6 mouse splenocytes labelling ICAM1 with ab25553 at 0.1 μg/106 cells and labelling CD49b with a Rat Anti-Mouse CD49b-FITC antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab25553 has been referenced in 4 publications.
- Qin L et al. The roles of CD11/CD18 and ICAM-1 in acute Pseudomonas aeruginosa-induced pneumonia in mice. J Immunol 157:5016-21 (1996). PubMed: 8943409
- Welder CA et al. Inhibition of cell adhesion by microspheres coated with recombinant soluble intercellular adhesion molecule-1. J Immunol 150:2203-10 (1993). PubMed: 8095510
- Horley KJ et al. Molecular cloning of murine intercellular adhesion molecule (ICAM-1). EMBO J 8:2889-96 (1989). PubMed: 2573511
- Takei F Inhibition of mixed lymphocyte response by a rat monoclonal antibody to a novel murine lymphocyte activation antigen (MALA-2). J Immunol 134:1403-7 (1985). PubMed: 2981916