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  1. Link

    pe-icam1-antibody-yn1174-ab25553.pdf

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Immunology Cell Type Markers CD Adhesion
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PE Anti-ICAM1 antibody [YN1/1.7.4] (ab25553)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (4)

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Flow Cytometry - PE Anti-ICAM1 antibody [YN1/1.7.4] (ab25553)

    Key features and details

    • PE Rat monoclonal [YN1/1.7.4] to ICAM1
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG2b

    You may also be interested in

    Protein
    Recombinant Human ICAM1 protein (ab151393)

    View more associated products

    Overview

    • Product name

      PE Anti-ICAM1 antibody [YN1/1.7.4]
      See all ICAM1 primary antibodies
    • Description

      PE Rat monoclonal [YN1/1.7.4] to ICAM1
    • Host species

      Rat
    • Conjugation

      PE. Ex: 488nm, Em: 575nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      The details of the immunogen for this antibody are not available.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.3
      Preservative: 0.09% Sodium azide
      Constituents: PBS, 16% Sucrose

      Also contains a stabilizing agent.
    • Concentration information loading...
    • Purity

      Affinity purified
    • Clonality

      Monoclonal
    • Clone number

      YN1/1.7.4
    • Isotype

      IgG2b
    • Light chain type

      kappa
    • Research areas

      • Immunology
      • Cell Type Markers
      • CD
      • Adhesion
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Leukocyte recruitment
      • Cell adhesion molecules
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Immunology
      • Adaptive Immunity
      • Regulatory T Cells
      • Cardiovascular
      • Angiogenesis
      • Endothelial Cell Markers

    Associated products

    • Alternative Versions

      • Anti-ICAM1 antibody [YN1/1.7.4] (ab119871)
      • FITC Anti-ICAM1 antibody [YN1/1.7.4] (ab24869)
      • Biotin Anti-ICAM1 antibody [YN1/1.7.4] (ab25007)
      • Anti-ICAM1 antibody [YN1/1.7.4] (ab25375)
      • APC Anti-ICAM1 antibody [YN1/1.7.4] (ab87026)
    • Isotype control

      • R-PE Rat IgG2b, kappa Monoclonal [KLH/G2b-1-2] - Isotype Control (ab136585)
    • Recombinant Protein

      • Recombinant Human ICAM1 protein (ab151393)

    Applications

    Our Abpromise guarantee covers the use of ab25553 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 0.2µg for 106 cells.

    Target

    • Function

      ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily. ICAM family.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    • Post-translational
      modifications

      Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P05362 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 15894 Mouse
      • SwissProt: P13597 Mouse
      • Unigene: 435508 Mouse
      • Alternative names

        • Antigen identified by monoclonal antibody BB2 antibody
        • BB 2 antibody
        • BB2 antibody
        • CD 54 antibody
        • CD_antigen=CD54 antibody
        • CD54 antibody
        • Cell surface glycoprotein P3.58 antibody
        • Human rhinovirus receptor antibody
        • ICAM 1 antibody
        • ICAM-1 antibody
        • ICAM1 antibody
        • ICAM1_HUMAN antibody
        • intercellular adhesion molecule 1 (CD54) antibody
        • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
        • Intercellular adhesion molecule 1 antibody
        • Major group rhinovirus receptor antibody
        • MALA 2 antibody
        • MALA2 antibody
        • MyD 10 antibody
        • MyD10 antibody
        • P3.58 antibody
        • Surface antigen of activated B cells antibody
        • Surface antigen of activated B cells, BB2 antibody
        see all

      Images

      • Flow Cytometry - PE Anti-ICAM1 antibody [YN1/1.7.4] (ab25553)
        Flow Cytometry - PE Anti-ICAM1 antibody [YN1/1.7.4] (ab25553)

        Flow cytometric analysis of C57BL/6 mouse splenocytes labelling ICAM1 with ab25553 at 0.1 μg/106 cells and labelling CD49b with a Rat Anti-Mouse CD49b-FITC antibody.

      Protocols

      • Flow cytometry protocols
      • Immunoprecipitation protocols
      • Immunohistochemistry protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (4)

      Publishing research using ab25553? Please let us know so that we can cite the reference in this datasheet.

      ab25553 has been referenced in 4 publications.

      • Qin L  et al. The roles of CD11/CD18 and ICAM-1 in acute Pseudomonas aeruginosa-induced pneumonia in mice. J Immunol 157:5016-21 (1996). PubMed: 8943409
      • Welder CA  et al. Inhibition of cell adhesion by microspheres coated with recombinant soluble intercellular adhesion molecule-1. J Immunol 150:2203-10 (1993). PubMed: 8095510
      • Horley KJ  et al. Molecular cloning of murine intercellular adhesion molecule (ICAM-1). EMBO J 8:2889-96 (1989). PubMed: 2573511
      • Takei F Inhibition of mixed lymphocyte response by a rat monoclonal antibody to a novel murine lymphocyte activation antigen (MALA-2). J Immunol 134:1403-7 (1985). PubMed: 2981916

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Flow Cytometry abreview for Anti-ICAM1 antibody [YN1/1.7.4] (Phycoerythrin)

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Mouse Cell (lymph node)
      Specification
      lymph node
      Preparation
      Cell harvesting/tissue preparation method: digestion collagenase
      Sample buffer: PBS/BSA 1%
      Permeabilization
      No
      Gating Strategy
      PI negative
      Read More

      Dr. alexandre garin

      Verified customer

      Submitted Jul 29 2008

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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