PE Anti-ICOS Ligand/ICOSL antibody [HK5.3] (ab93551)
![Flow Cytometry - PE Anti-ICOS Ligand/ICOSL antibody [HK5.3] (ab93551)](https://www.abcam.com/ps/products/93/ab93551/Images/ab93551-108584-anti-icos-ligand-antibody-hk53-phycoerythrin-flow-cytometry.gif "Flow Cytometry - PE Anti-ICOS Ligand/ICOSL antibody [HK5.3] (ab93551)")
Key features and details
- PE Rat monoclonal [HK5.3] to ICOS Ligand/ICOSL
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Overview
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Product name
PE Anti-ICOS Ligand/ICOSL antibody [HK5.3]
See all ICOS Ligand/ICOSL primary antibodies -
Description
PE Rat monoclonal [HK5.3] to ICOS Ligand/ICOSL -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse ICOS Ligand/ICOSL.
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Positive control
- BALB/c splenocytes.
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General notes
This product was previously labelled as ICOS Ligand
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
HK5.3 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab93551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. -
Tissue specificity
Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leukocytes and spleen. Expressed on activated monocytes and dendritic cells. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 50723 Mouse
- SwissProt: Q9JHJ8 Mouse
- Unigene: 17819 Mouse
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Alternative names
- B7 H2 antibody
- B7 homolog 2 antibody
- B7 homologue 2 antibody
see all
Images
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Flow Cytometry - PE Anti-ICOS Ligand/ICOSL antibody [HK5.3] (ab93551)
ab93551 at 0.06µg dilution staining ICOS Ligand/ICOSL in BALB/c splenocytes by Flow Cytometry.
Datasheets and documents
References (0)
ab93551 has not yet been referenced specifically in any publications.
